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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Identification of Potential Diagnostic and Vaccine Candidates ofHelicobacter pylori by Two-Dimensional Gel Electrophoresis, Sequence Analysis, and Serum Profiling
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Identification of Potential Diagnostic and Vaccine Candidates ofHelicobacter pylori by Two-Dimensional Gel Electrophoresis, Sequence Analysis, and Serum Profiling

机译：通过二维凝胶电泳，序列分析和血清分析鉴定幽门螺杆菌的潜在诊断和疫苗候选者

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摘要

There is great interest in characterizing the proteins of the gastric pathogen Helicobacter pylori, especially those to which humans respond immunologically, because of the potential importance of such proteins in diagnosis and vaccine development. Two-dimensional gel electrophoresis was used to separate and identify potential antigens of H. pylori ATCC 43504. Over 30 proteins were reactive in Western blots with pooled sera from 14 infected patients. These proteins were analyzed by N-terminal sequence analysis. Fourteen proteins were determined to be distinct from any proteins previously described from H. pylori; the others were previously isolated and characterized proteins. Analysis of eight distinct H. pylori strains showed that most of these antigens were produced by all of the strains. We propose that collection of new antigens such as those recognized here will be useful in serologic tests for detecting and monitoring H. pylori infection and may also serve as potential targets for antimicrobial agent or vaccine development.
机译：人们对表征胃病原体幽门螺杆菌（Helicobacter pylori）的蛋白质非常感兴趣，尤其是人类免疫应答的蛋白质，因为这种蛋白质在诊断和疫苗开发中具有潜在的重要性。二维凝胶电泳用于分离和鉴定 H的潜在抗原。 pylori ATCC43504。有超过30种蛋白质在Western印迹中与14例受感染患者的血清反应。通过N端序列分析来分析这些蛋白质。测定了十四种蛋白质与先前从 H描述的任何蛋白质不同。幽门螺杆菌;其他的则是先前分离和鉴定的蛋白质。分析八个不同的 H。幽门螺杆菌菌株显示大多数这些抗原是由所有菌株产生的。我们建议收集新抗原（例如此处识别的抗原）将用于血清学检测和监测H中。幽门螺杆菌感染，也可能成为抗菌剂或疫苗开发的潜在目标。

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