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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Interleukin 7 Can Enhance Antigen-Specific Cytotoxic-T-Lymphocyte and/or Th2-Type Immune Responses In Vivo
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Interleukin 7 Can Enhance Antigen-Specific Cytotoxic-T-Lymphocyte and/or Th2-Type Immune Responses In Vivo

机译：白介素7可以增强抗原特异性的细胞毒性T淋巴细胞和/或体内的Th2型免疫反应。

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Interleukin 7 (IL-7) protein has been reported to be important in the development of cytotoxic-T-lymphocyte (CTL) responses. However, other studies also support a partial Th2 phenotype for this cytokine. In an effort to clarify this unusual conflict, we compared IL-7 along with IL-12 (Th1 control) and IL-10 (Th2 control) for its ability to induce antigen (Ag)-specific CTL and Th1- versus Th2-type immune responses using a well established DNA vaccine model. In particular, IL-7 codelivery showed a significant increase in immunoglobulin G1 (IgG1) levels compared to IgG2a levels. IL-7 coinjection also decreased production of Th1-type cytokine IL-2, gamma interferon, and the chemokine RANTES but increased production of the Th2-type cytokine IL-10 and the similarly biased chemokine MCP-1. In herpes simplex virus (HSV) challenge studies, IL-7 coinjection decreased the survival rate after lethal HSV type 2 (HSV-2) challenge compared with gD plasmid vaccine alone in a manner similar to IL-10 coinjection, whereas IL-12 coinjection enhanced the protection, further supporting that IL-7 drives immune responses to the Th2 type, resulting in reduced protection against HSV-2 challenge. Moreover, coinjection with human immunodeficiency virus type 1 env and gag/polgenes plus IL-12 or IL-7 cDNA enhanced Ag-specific CTLs, while coinjection with IL-10 cDNA failed to influence CTL induction. Thus, IL-7 could drive Ag-specific Th2-type cellular responses and/or CTL responses. These results support that CTLs could be induced by IL-7 in a Th2-type cytokine and chemokine environment in vivo. This property of IL-7 allows for an alternative pathway for CTL development which has important implications for host-pathogen responses.
机译：据报道，白介素7（IL-7）蛋白在细胞毒性T淋巴细胞（CTL）反应的发展中很重要。但是，其他研究也支持该细胞因子的部分Th2表型。为了澄清这种不寻常的冲突，我们比较了IL-7，IL-12（Th1对照）和IL-10（Th2对照）诱导抗原（Ag）特异性CTL和Th1-Th2型的能力。使用完善的DNA疫苗模型进行免疫反应。特别是，与IgG2a水平相比，IL-7代码传递显示免疫球蛋白G1（IgG1）水平显着增加。 IL-7共注射还降低了Th1型细胞因子IL-2，γ干扰素和趋化因子RANTES的产生，但增加了Th2型细胞因子IL-10和类似偏向的趋化因子MCP-1的产生。在单纯疱疹病毒（HSV）攻击研究中，与单独使用gD质粒疫苗相比，IL-7共注射降低了致死性2型HSV（HSV-2）攻击后的存活率，而IL-12共注射增强了保护作用，进一步支持IL-7可以驱动针对Th2型的免疫反应，从而降低了针对HSV-2攻击的保护作用。此外，与人类免疫缺陷病毒1型 env 和 gag / pol 基因加IL-12或IL-7 cDNA共注射增强了Ag特异性CTL，而与IL-10共注射cDNA无法影响CTL的诱导。因此，IL-7可以驱动Ag特异性的Th2型细胞应答和/或CTL应答。这些结果支持IL-7可在体内Th2型细胞因子和趋化因子环境中诱导CTL。 IL-7的这一特性为CTL的发展提供了另一种途径，这对宿主-病原体反应具有重要意义。

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