Cholera Toxin B-Subunit Gene Enhances Mucosal Immunoglobulin A, Th1-Type, and CD8+ Cytotoxic Responses When Coadministered Intradermally with a DNA Vaccine

Alba E. Sanchez; Guillermo Aquino; Pedro Ostoa-Saloma; Juan P. Laclette; Leticia Rocha-Zavaleta

首页> 外文期刊>Clinical and diagnostic laboratory immunology >Cholera Toxin B-Subunit Gene Enhances Mucosal Immunoglobulin A, Th1-Type, and CD8+ Cytotoxic Responses When Coadministered Intradermally with a DNA Vaccine

【24h】

Cholera Toxin B-Subunit Gene Enhances Mucosal Immunoglobulin A, Th1-Type, and CD8+ Cytotoxic Responses When Coadministered Intradermally with a DNA Vaccine

机译：霍乱毒素B亚基基因与DNA疫苗一起皮内给药可增强粘膜免疫球蛋白A，Th1型和CD8 +细胞毒性反应

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

A plasmid vector encoding the cholera toxin B subunit (pCtB) was evaluated as an intradermal genetic adjuvant for a model DNA vaccine expressing the human papillomavirus type 16 L1 capsid gene (p16L1) in mice. p16L1 was coadministered with plasmid pCtB or commercial polypeptide CtB as a positive control. Coadministration of pCtB induced a significant increment of specific anti-L1 immunoglobulin A (IgA) antibodies in cervical secretions (P < 0.05) and fecal extracts (P < 0.005). Additionally, coadministration of pCtB enhanced the production of interleukin-2 and gamma interferon by spleen cells but did not affect the production of interleukin-4, suggesting a Th1-type helper response. Furthermore, improved CD8+ T-cell-mediated cytotoxic activity was observed in mice vaccinated with the DNA vaccine with pCtB as an adjuvant. This adjuvant effect was comparable to that induced by the CtB polypeptide. These results indicate that intradermal coadministration of pCtB is an adequate means to enhance the mucosa-, Th1-, and CD8+-mediated cytotoxic responses induced by a DNA vaccine.

机译：编码霍乱毒素B亚基（pCtB）的质粒载体被评估为在小鼠中表达人乳头瘤病毒16型L1衣壳基因（p16L1）的模型DNA疫苗的皮内遗传佐剂。将p16L1与质粒pCtB或商业多肽CtB共同给药作为阳性对照。 pCtB的共同给药在宫颈分泌物（ P <0.05）和粪便提取物（ P <0.005）中诱导特异性的抗L1免疫球蛋白A（IgA）抗体显着增加。此外，pCtB的共同给药可增强脾细胞产生白介素2和γ干扰素，但不影响白介素4的产生，提示Th1型辅助应答。此外，在接种了以pCtB为佐剂的DNA疫苗的小鼠中，观察到了CD8 + T细胞介导的细胞毒性活性的改善。该佐剂作用与CtB多肽诱导的佐剂作用相当。这些结果表明，皮内共同施用pCtB是增强DNA疫苗诱导的粘膜，Th1-和CD8 + 介导的细胞毒性反应的适当手段。 展开▼

著录项

来源
《Clinical and diagnostic laboratory immunology》 |2004年第4期|共9页

作者
Alba E. Sanchez; Guillermo Aquino; Pedro Ostoa-Saloma; Juan P. Laclette; Leticia Rocha-Zavaleta;
展开▼

作者单位

展开▼

收录信息

原文格式 PDF

正文语种

中图分类诊断学;

关键词

相似文献

外文文献

中文文献

专利

1. Heteropentameric Cholera Toxin B Subunit Chimeric Molecules Genetically Fused to a Vaccine Antigen Induce Systemic and Mucosal Immune Responses: a Potential New Strategy To Target Recombinant Vaccine Antigens to Mucosal Immune Systems [J] . Tetsuya Harakuni, Hideki Sugawa, Ai Komesu, Infection and immunity . 2005,第9期

机译：遗传融合到疫苗抗原的异源性霍乱毒素B亚基嵌合分子诱导全身和粘膜免疫反应：靶向重组疫苗抗原对粘膜免疫系统的潜在新策略。

2. Heteropentameric Cholera Toxin B Subunit Chimeric Molecules Genetically Fused to a Vaccine Antigen Induce Systemic and Mucosal Immune Responses: a Potential New Strategy To Target Recombinant Vaccine Antigens to Mucosal Immune Systems [J] . Tetsuya Harakuni, Hideki Sugawa, Ai Komesu, Infection and immunity . 2005,第9期

机译：遗传融合到疫苗抗原的异源性霍乱毒素B亚基嵌合分子诱导全身和粘膜免疫反应：靶向重组疫苗抗原对粘膜免疫系统的潜在新策略。

3. Live varicella vaccine polarizes the mucosal adjuvant action of cholera toxin or its B subunit on specific Th1-type helper T cells with a single nasal coadministration in mice. [J] . Sasaki K, Kato M, Takahashi T, Journal of Medical Virology . 2003,第2期

机译：水痘活疫苗通过一次鼻腔共同给药使霍乱毒素或其B亚基对特定Th1型辅助T细胞的粘膜佐剂作用极化。

4. INTRADERMAL ADMINISTRATION OF SYNTHETIC DNA VACCINES INDUCE ROBUST CELLULAR AND HUMORAL IMMUNE RESPONSES [C] . Jean D. Boyer Conference on vaccine technology VI . 2018

机译：皮内管理合成DNA疫苗诱导强健的细胞和体液免疫反应

5. Factors influencing function: Activation of antigen presenting cells by the cholera toxin B-subunit, and regulation of the cholinergic phenotype in nerve growth factor receptor-positive basal forebrain cholinergic neurons by bone morphogenetic protein 9. [D] . Schnitzler, Aletta Christina. 2007

机译：影响功能的因素：霍乱毒素B亚基激活抗原呈递细胞，并通过骨形态发生蛋白9调节神经生长因子受体阳性基底前脑胆碱能神经元的胆碱能表型。

6. Cholera Toxin B-Subunit Gene Enhances Mucosal Immunoglobulin A Th1-Type and CD8+ Cytotoxic Responses When Coadministered Intradermally with a DNA Vaccine [O] . Alba E. Sanchez, Guillermo Aquino, Pedro Ostoa-Saloma, 2004

机译：霍乱毒素B亚基基因与DNA疫苗一起皮内给药可增强粘膜免疫球蛋白ATh1型和CD8 +细胞毒性反应

7. Cholera Toxin B-Subunit Gene Enhances Mucosal Immunoglobulin A, Th1-Type, and CD8+ Cytotoxic Responses When Coadministered Intradermally with a DNA Vaccine [O] . Sanchez, Alba E., Aquino, Guillermo, Ostoa-Saloma, Pedro, 2004

机译：霍乱毒素B亚基基因与DNA疫苗一起皮内给药可增强粘膜免疫球蛋白A，Th1型和CD8 +细胞毒性反应

1. 霍乱毒素B亚基与疟原虫多表位构建的DNA疫苗与亚单位疫苗的免疫保护作用研究 [J] . 钟辉 ,曹诚 ,等 . 中国药理学会通讯 . 2000,第003期

2. 以恒河猴为模型的霍乱毒素B亚基与疟原虫多表位构建的DNA疫苗的免疫保护作用 [J] . 钟辉 ,曹诚 ,李平 . 军事医学 . 2000,第002期

3. 人乳头瘤病毒11型DNA疫苗协同CD80基因诱导小鼠特异性免疫反应 [J] . 李莉华 ,黄朝晖 ,郭子健 . 中华皮肤科杂志 . 2006,第012期

4. 霍乱肠毒素基因DNA疫苗的构建和体外表达 [J] . 张莉 ,张雷 ,陈建平 . 西部医学 . 2007,第005期

5. 大肠杆菌LTB亚基基因表达载体对犬细小病毒VP2 DNA疫苗免疫应答的增强作用 [J] . 韩冬梅 ,仲飞 ,李秀锦 . 微生物学报 . 2011,第001期

6. 共表达霍乱毒素B亚基的DNA疫苗的系统及粘膜佐剂活性研究 [A] . 邱苏赣 . 2013

1. RECOMBINANT PLASMIDE DNA P1EM3 ENCODING SYNTHESIS OF B-SUBUNIT OF CHOLERA TOXIN, METHOD OF ITS CONSTRUCTING, AND STRAIN OF VIBRIO CHOLERAE BACTERIA PRODUCING B-SUBUNIT OF CHOLERA TOXIN [P] . 外国专利： SU1505022A1 . 1991-10-15

机译：编码霍乱毒素B亚基的重组质粒DNA P1EM3的合成，构建方法及产霍乱弧菌B-亚基的霍乱弧菌细菌的生产

2. Oral vaccine against enterotoxigenic E. coli comprising at least three different types of colonisation factor antigens on killed and modified E coli and the B-subunit of cholera toxin [P] . 外国专利： NZ527559A . 2005-01-28

机译：抗肠毒素大肠杆菌的口服疫苗，在已杀死和修饰的大肠杆菌上含有至少三种不同类型的定殖因子抗原和霍乱毒素的B亚基

3. Mutant immunogenic cholera holotoxin, immunogenic composition, method for enhancing a vertebrate host's immune response to an antigen, isolated and purified DNA sequence, nucleic acid molecule, host cell, method for producing a mutant immunogenic cholera holotoxin, and, use of a mutant cholera holotoxin [P] . 外国专利： BR0210225A . 2004-09-08

机译：突变的免疫霍乱全毒素，免疫原性组合物，增强脊椎动物宿主对抗原的免疫反应的方法，分离和纯化的DNA序列，核酸分子，宿主细胞，生产突变的免疫霍乱全毒素的方法以及突变霍乱全毒素的用途

相关主题

Cholera Toxin B-Subunit Gene Enhances Mucosal Immunoglobulin A, Th1-Type, and CD8+ Cytotoxic Responses When Coadministered Intradermally with a DNA Vaccine

摘要

著录项

相似文献

相关主题

期刊订阅