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首页> 外文期刊>Clinical and diagnostic laboratory immunology >vacA Genotypes and Genetic Diversity in Clinical Isolates of Helicobacter pylori
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vacA Genotypes and Genetic Diversity in Clinical Isolates of Helicobacter pylori

机译：幽门螺杆菌临床分离株的vacA基因型和遗传多样性

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Genetic diversity in Helicobacter pylori strains may affect the function and antigenicity of virulence factors associated with bacterial infection and, ultimately, disease outcome. In this study, DNA diversity of H. pylori isolates was examined by analysis of vacA genotypes and by restriction fragment length polymorphism (RFLP) analysis of H. pylori-associated genes (vacA, cagA, flaA,ureAB, and ureCD). Thirty-seven H. pylori isolates from 26 patients were successfully classified into distinct vacA allelic genotypes. The signal sequence allele s1 (31 of 37) predominated over the s2 allele (6 of 37) and was significantly associated with the occurrence (past or present) of gastric ulcers. A novel midregion allele, designated as m3, has been identified in two H. pylori isolates which could not be typed with midregion allele m1- or m2-specific primers. Additionally, significant nucleotide diversity yielding different amino acid sequences was demonstrated by DNA sequencing of vacAfragments from clinical isolates of H. pylori. Furthermore, RFLP analysis of 45 H. pylori isolates (including 15 paired isolates) obtained from antrum and corpus biopsy specimens from 30 individual patients showed remarkably high interhost diversity (one patient, one H. pylori strain) and intrahost identity in gene sequences coding for VacA, CagA, flagellin, and urease. Only in a single patient was a minor genotypic variation at different anatomic sites within the stomach identified. These data warrant the detailed analysis of the effect of genetic diversity on the function and antigenicity of H. pylori-associated virulence factors.
机译：幽门螺杆菌菌株的遗传多样性可能会影响与细菌感染有关的毒力因子的功能和抗原性，并最终影响疾病结局。在这项研究中， H的DNA多样性。通过分析 vacA 基因型和 H的限制性片段长度多态性（RFLP）分析检查幽门螺杆菌。幽门螺杆菌相关基因（ vacA ， cagA ， flaA ， ureAB 和 ureCD ）。三十七小时将26例患者的幽门螺杆菌分离株成功地分为不同的 vacA 等位基因型。信号序列等位基因s1（37中的31）高于s2等位基因（37中的6），并且与胃溃疡的发生（过去或现在）显着相关。在两个 H中鉴定出一个新的中部区域等位基因，命名为m3。不能用中等区域等位基因m1或m2特异性引物分型的幽门螺杆菌分离株。此外，通过对 H临床分离株的 vacA 片段进行DNA测序，证明了产生不同氨基酸序列的显着核苷酸多样性。幽门炎。此外，对45 H的RFLP分析。从30例患者的胃窦和尸体活检标本中获得的幽门螺杆菌分离株（包括15对配对株）显示出极高的宿主间多样性（一名患者，一种幽门螺杆菌菌株）和该基因的宿主内同一性编码VacA，CagA，鞭毛蛋白和脲酶的序列。仅在一名患者中，在胃内不同解剖部位发现了较小的基因型变异。这些数据保证了遗传多样性对 H的功能和抗原性的影响的详细分析。幽门螺杆菌相关毒力因子

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