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首页> 外文期刊>Clinical and diagnostic laboratory immunology >A Whole-Blood Assay for Qualitative and Semiquantitative Measurements of CD69 Surface Expression on CD4 and CD8 T Lymphocytes Using Flow Cytometry
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A Whole-Blood Assay for Qualitative and Semiquantitative Measurements of CD69 Surface Expression on CD4 and CD8 T Lymphocytes Using Flow Cytometry

机译：使用流式细胞术对CD4和CD8 T淋巴细胞上CD69表面表达进行定性和半定量测量的全血分析

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A whole-blood flow cytometry-based assay was utilized to assess CD4 and CD8 T-lymphocyte activation in response to phytohemagglutinin (PHA) stimulation. T-lymphocyte activation was assessed by qualitative (percent CD69) and semiquantitative (anti-CD69 antibody binding capacity) measurements of CD69 surface expression. Whole-blood samples from 21 healthy and 21 human immunodeficiency virus (HIV)-infected (<500 absolute CD4 counts per mm3) individuals were stimulated with 20 μg of PHA per ml for 18 to 24 h. The proportions of activated CD4 and CD8 T lymphocytes expressing CD69 (percent CD69) and the levels of CD69 expression on each T-lymphocyte subset (anti-CD69 antibody binding capacity) were measured. By using this assay system, T-lymphocyte activation was impaired in both CD4 and CD8 T-lymphocyte subsets of HIV-infected individuals. The proportions of CD69-positive CD4 and CD8 T lymphocytes were 43 and 27% lower, respectively, in samples from HIV-infected individuals compared to samples from healthy individuals. Similarly, the levels of CD69 expression on each activated CD4 and CD8 T-lymphocyte subset were 48 and 51% lower, respectively. These results suggest that both qualitative and semiquantitative measurements of CD69 surface expression by flow cytometry can be used to assess T-lymphocyte activation.
机译：基于全血流式细胞术的测定法用于评估响应植物血凝素（PHA）刺激的CD4和CD8 T淋巴细胞活化。通过对CD69表面表达的定性（CD69百分比）和半定量（抗CD69抗体结合能力）测量来评估T淋巴细胞的活化。用每毫升20μgPHA刺激21例健康人和21例人类免疫缺陷病毒（HIV）感染（<500绝对CD4计数/ mm 3 ）个体的全血样品，刺激时间为18-24小时。测量表达CD69的活化CD4和CD8 T淋巴细胞的比例（百分比CD69）以及每个T淋巴细胞亚群上CD69表达的水平（抗CD69抗体结合能力）。通过使用此检测系统，HIV感染者的CD4和CD8 T淋巴细胞亚群中的T淋巴细胞活化均受到损害。与健康人的样本相比，HIV感染者的样本中CD69阳性CD4和CD8 T淋巴细胞的比例分别降低了43％和27％。同样，每个激活的CD4和CD8 T淋巴细胞亚群上的CD69表达水平分别降低了48％和51％。这些结果表明通过流式细胞术对CD69表面表达的定性和半定量测量均可用于评估T淋巴细胞的活化。

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