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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Diversity of Hemagglutination Phenotypes among P-Fimbriated Wild-Type Strains of Escherichia coli in Relation to papG Allele Repertoire
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Diversity of Hemagglutination Phenotypes among P-Fimbriated Wild-Type Strains of Escherichia coli in Relation to papG Allele Repertoire

机译：与pG等位基因库相关的大肠杆菌P型野生型血凝表型的血凝表型多样性

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Data regarding the hemagglutination (HA) patterns of the three variants (classes I, II, and III) of the Escherichia coliadhesin PapG are conflicting. These HA patterns usually have been assessed for each papG allele separately with recombinant strains in slide HA assays. We rigorously evaluated an alternative microtiter tray HA assay and then used it to assess the HA of four erythrocyte types (human A1P1 and OP1, rabbit, and sheep erythrocytes) by multiple wild-typeE. coli strains representing the four naturally occurring combinations of the papG alleles, i.e., class I plus III, class III only, class II plus III, and class II only. The microtiter tray HA assay displayed significantly better reproducibility of intraobserver (83%) and interobserver (86%) results than did slide HA assays (39 and 73%, respectively). Novel findings from the study of 32 wild-type P-fimbriated strains included reproducible determinations of phenotypic diversity among different papG categories, among strains within each papG category, and from day to day for individual strains. There was also substantial overlap of phenotypes between papG categories I plus III and III only and between II plus III and II only. A class III papG recombinant strain’s HA pattern differed significantly from that of the wild-type class III strains. These data demonstrate that HA phenotypes of wild-type P-fimbriated E. coli strains can be reproducibly assessed by a microtiter HA assay and that they correspond broadly topapG genotype but in a more complex and varied fashion than previously recognized.
机译：关于大肠杆菌粘附素PapG的三个变体（I，II和III类）的血凝（HA）模式的数据相互矛盾。通常在玻片HA分析中用重组菌株分别评估每个 papG 等位基因的这些HA模式。我们严格评估了另一种微量滴定盘HA检测方法，然后将其用于评估四种红细胞类型（人A 1 P 1 和OP 1 >，兔和绵羊的红血球）。代表 papG 等位基因的四个天然组合的大肠杆菌菌株，即I类加III，仅III类，II加III和仅II类。微量滴定板HA分析显示的观察者内（83％）和观察者间（86％）结果的重现性比载玻片HA分析（分别为39％和73％）更好。从对32种野生型P纤维菌株的研究中得出的新发现包括可重复测定不同的 papG 类别之间，每个 papG 类别中的菌株之间的表型多样性。个别菌株的一天。在 papG 类别I加III和III之间以及仅II加III与II之间，表型也存在大量重叠。 III类 papG 重组菌株的HA模式与野生型III类菌株显着不同。这些数据证明野生型P-成纤维的 E的HA表型。可以通过微量滴定HA分析可重复地评估大肠杆菌菌株，它们与 papG 基因型广泛对应，但是比以前公认的更为复杂和多样。

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