Differentiation of F18ab+ from F18ac+ Escherichia coli by Single-Strand Conformational Polymorphism Analysis of the Major Fimbrial Subunit Gene (fedA)

Brad T. Bosworth; Evelyn A. Dean-Nystrom; Thomas A. Casey; Holly L. Neibergs

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Differentiation of F18ab+ from F18ac+ Escherichia coli by Single-Strand Conformational Polymorphism Analysis of the Major Fimbrial Subunit Gene (fedA)

机译：通过主要纤维亚基基因（fedA）的单链构象多态性分析从F18ac +大肠杆菌中区分出F18ab +。

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Toxin-producing Escherichia coli expressing F18 fimbriae colonizes the small intestines of weaned pigs and causes diarrhea, edema disease, or both. The F18 family is composed of two antigenic variants, F18ab and F18ac. Because many strains do not express F18 fimbriae in vitro, identification and differentiation of these two variants are difficult. Single-strand conformational polymorphism (SSCP) analysis is a rapid method for identifying genetic mutations and polymorphisms. The F18 major fimbrial subunit genes (fedA) of 138 strains were amplified by PCR, and genetic differences were detected by SSCP analysis. The SSCP analysis of thefedA gene differentiated F18ab+ strains from F18ac+ strains. Most strains classified as F18ab+ by SSCP analysis contained Shiga toxin 2e and enterotoxin genes. Most strains classified as F18ac+ by SSCP analysis contained only enterotoxin genes. The SSCP analysis was a useful method for predicting the antigenicity of F18+ E. coli and could also be used for analysis of other virulence genes in E. coli and other pathogenic bacteria.

机译：表达F18菌毛的产毒素大肠杆菌表达在断奶猪的小肠内定殖，引起腹泻，水肿或两者兼有。 F18家族由两个抗原变体F18ab和F18ac组成。由于许多菌株在体外不表达F18菌毛，因此很难鉴定和区分这两个变异体。单链构象多态性（SSCP）分析是鉴定遗传突变和多态性的快速方法。 PCR扩增138个菌株的F18主要纤维亚基基因（ fedA ），并通过SSCP分析检测其遗传差异。对 fedA 基因从F18ac + 菌株中分离出的F18ab + 菌株进行SSCP分析。通过SSCP分析归类为F18ab + 的大多数菌株均包含志贺毒素2e和肠毒素基因。通过SSCP分析归类为F18ac + 的大多数菌株仅包含肠毒素基因。 SSCP分析是预测F18 + E抗原性的有用方法。大肠杆菌，也可用于分析 E中的其他毒力基因。大肠杆菌和其他致病菌。 展开▼

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《Clinical and diagnostic laboratory immunology》 |1998年第3期|共4页

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Brad T. Bosworth; Evelyn A. Dean-Nystrom; Thomas A. Casey; Holly L. Neibergs;
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中图分类诊断学;

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专利

1. Characterization of F107 fimbriae of Escherichia coli 107/86, which causes edema disease in pigs, and nucleotide sequence of the F107 major fimbrial subunit gene, fedA. [J] . H Imberechts, H De Greve, C Schlicker, Infection and immunity . 1992,第5期

机译：导致猪水肿的大肠杆菌107/86 F107菌毛的特征以及F107主要纤维亚基基因fedA的核苷酸序列。

2. Hydrophobic cluster analysis and secondary structure predictions revealed that major and minor structural subunits of K88‐related adhesins of Escherichia coli share a common overall fold and differ structurally from other fimbrial subunits [J] . Bertin Yolande, Girardeau Jean-Pierre, Meacute, FEBS Letters . 1995,第3期

机译：疏水簇分析和二级结构预测表明，大肠杆菌K88相关粘附素的主要和次要结构亚基共享相同的整体折叠，并且在结构上与其他纤维亚基不同

3. Construction of Bifidobacterium infantis as a live oral vaccine that expresses antigens of the major fimbrial subunit (CfaB) and the B subunit of heat-labile enterotoxin (LTB) from enterotoxigenic Escherichia coli [J] . Yongping Ma, Yaolin Luo, Xueping Huang, Microbiology . 2012,第2期

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4. DNA POLYMORPHISMS GENERATED BY SINGLE-STRAND CONFORMATIONAL POLYMORPHISM AND RANDOM AMPLIFIED POLYMORPHIC DNA TECHNIQUE ARE USEFUL AS TOOLS FOR THAI VETIVER GENOME ANALYSIS [C] . . 2003

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5. Molecular characterization and expression of Salmonella enterica serotype Enteritidis fimbrial genes in Escherichia coli. [D] . Lopes-Berkas, Vanessa Cristina. 2006

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6. Differentiation of F18ab+ from F18ac+ Escherichia coli by Single-Strand Conformational Polymorphism Analysis of the Major Fimbrial Subunit Gene (fedA) [O] . Brad T. Bosworth, Evelyn A. Dean-Nystrom, Thomas A. Casey, 1998

机译：通过主要纤维亚基基因（fedA）的单链构象多态性分析从F18ac +大肠杆菌中区分出F18ab +。

7. Differentiation of F18ab + from F18ac + Escherichia coli by Single-Strand Conformational Polymorphism Analysis of the Major Fimbrial Subunit Gene ( fedA ) [O] . Brad T. Bosworth, Evelyn A. Dean-Nystrom, Thomas A. Casey, 1998

机译：通过单链构象多态性分析（FEDA）单链构象多态性分析（FEDA）的单链构象多态性分析，F18ab +从F18AB +大肠杆菌分化

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Differentiation of F18ab+ from F18ac+ Escherichia coli by Single-Strand Conformational Polymorphism Analysis of the Major Fimbrial Subunit Gene (fedA)

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