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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Serodiagnosis of Neosporosis in Individual Cows and Dairy Herds: A Comparative Study of Three Enzyme-Linked Immunosorbent Assays
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Serodiagnosis of Neosporosis in Individual Cows and Dairy Herds: A Comparative Study of Three Enzyme-Linked Immunosorbent Assays

机译：牛和奶牛新孢子虫病的血清学诊断：三种酶联免疫吸附法的比较研究

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The performance of three enzyme-linked immunosorbent assays (ELISA) for detection of antibodies to Neospora caninum in bovine sera was evaluated by using various categories of sera. Two commercial ELISA methods, one based on chemically fixed intact tachyzoites and one based on a sonicate lysate of whole tachyzoites, were compared with an in-house ELISA based on a detergent lysate of whole tachyzoites. A brief description of the development of the latter ELISA is also given. There was good agreement among all three tests with regard to postabortion sera. By using acute-phase abortion sera from cows with confirmedN. caninum-induced and non-N. caninum-induced abortions, satisfactory levels of sensitivity and specificity were calculated for all tests. In addition, similar test results were obtained with postpartum samples from dams and calves. However, considerable differences were found between test results of sequential samples and cross-sectional and total-herd samples. Apparently, these discrepancies were due to different sensitivities of the tests for detection of low antibody levels in chronically infected animals. It is suggested that these differences were primarily due to the use of different antigens and different test sample dilutions. It is concluded that all tests are applicable as an additional diagnostic tool in cases of abortion in cattle and for monitoring of congenitally infected calves. For herd screening, the lysate-based ELISAs appear to be more adequate because of their higher sensitivities.
机译：使用各种血清评估了三种酶联免疫吸附试验（ELISA）在牛血清中检测犬新孢子虫抗体的性能。比较了两种商业化ELISA方法，一种基于化学固定的完整速殖子，一种基于全速殖子的超声裂解物，而另一种是基于内部ELISA的全速殖子去污剂裂解物。还简要介绍了后一种ELISA的发展。在所有三个关于流产后血清的测试中都有很好的一致性。通过使用确诊为em的奶牛的急性期流产血清。犬诱导的和非 N。犬流产后，所有测试的敏感性和特异性均达到令人满意的水平。此外，从大坝和小牛的产后样品获得了相似的测试结果。但是，在顺序样本与横截面样本和全牧群样本的测试结果之间发现了相当大的差异。显然，这些差异是由于在慢性感染动物中检测低抗体水平的测试灵敏度不同所致。建议这些差异主要是由于使用了不同的抗原和不同的测试样品稀释度。结论是，所有测试都可作为牛流产和监测先天感染牛犊的附加诊断工具。对于畜群筛选，基于裂解物的ELISA灵敏度更高，因此似乎更为合适。

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