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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Evaluation of the In Vitro Pyrogen Test System Based on Proinflammatory Cytokine Release from Human Monocytes: Comparison with a Human Whole Blood Culture Test System and with the Rabbit Pyrogen Test
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Evaluation of the In Vitro Pyrogen Test System Based on Proinflammatory Cytokine Release from Human Monocytes: Comparison with a Human Whole Blood Culture Test System and with the Rabbit Pyrogen Test

机译：基于人单核细胞促炎细胞因子释放的体外热原测试系统的评估：与人全血培养测试系统和兔热原测试的比较

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The reliability of an in vitro pyrogen test system based on proinflammatory cytokine release from human monocytic cells was assessed by comparison with a test system based on a human whole blood culture as well as with the conventional rabbit pyrogen test. The human cells used as the pyrogen indicator cells were newly selected by subcloning of a human monocytic cell line, Mono-Mac-6. The selected cells, named MM6-CA8, responded to various pyrogens, including endotoxin, peptidoglycan (PG), Staphylococcus aureus Cowan 1 (SAC), and poly(I?·??C), with a high sensitivity and produced proinflammatory cytokines, such as interleukin 1 (IL-1), IL-6, and tumor necrosis factor alpha. Among these cytokines, IL-6 was produced most sensitively in response to traces of the pyrogens and detected in the largest quantities in the culture medium. The cytokine-producing responses of MM6-CA8 cells correlated significantly with the responses of cultured human whole blood, which represents an ex vivo culture test system reproducing pyrogen-induced cytokine production in the human body. In terms of cytokine inducibility, the pyrogens were ranked in the order endotoxin > PG > poly (I?·?C) > SAC in both culture systems, a ranking which almost agreed with the ranking of their pyrogenicity as assessed by the rabbit pyrogen test. These results suggest that the in vitro responsiveness of MM6-CA8 cells to various pyrogens is highly relevant for human pyrogenic reactions. Therefore, the in vitro test system is useful and reliable for detecting the presence of materials that are pyrogenic for humans.
机译：通过与基于人全血培养物的测试系统以及常规兔热原测试进行比较，评估了基于从人单核细胞释放促炎细胞因子的体外热原测试系统的可靠性。通过亚克隆人单核细胞系Mono-Mac-6，新选择用作热原指示细胞的人细胞。选定的名为MM6-CA8的细胞对多种热原有反应，包括内毒素，肽聚糖（PG），金黄色葡萄球菌（SAC）Cowan 1（SAC）和聚（I？·?? C）。高敏感性并产生促炎细胞因子，例如白介素1（IL-1），IL-6和肿瘤坏死因子α。在这些细胞因子中，IL-6是对热原的痕迹最敏感地产生的，并且在培养基中含量最高。 MM6-CA8细胞的细胞因子产生反应与培养的人类全血反应显着相关，这代表了一种体外培养测试系统，可在人体内重生热原诱导的细胞因子产生。就细胞因子的诱导性而言，在两种培养系统中，热原的排列顺序依次为内毒素> PG>聚（I·？C）> SAC，该排名几乎与通过兔热原试验评估的其热原性排名一致。这些结果表明，MM6-CA8细胞对各种热原的体外反应性与人的热原反应高度相关。因此，体外测试系统对于检测对人类有致热性的物质的存在是有用且可靠的。

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