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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Development and Evaluation of an In-House Enzyme-Linked Immunosorbent Assay for Early Diagnosis and Monitoring of Human Pythiosis
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Development and Evaluation of an In-House Enzyme-Linked Immunosorbent Assay for Early Diagnosis and Monitoring of Human Pythiosis

机译：内部酶联免疫吸附测定方法的开发和评估，可用于早期诊断和监测人的烟毒病

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Human pythiosis is an emerging, fatal, infectious disease caused by Pythium insidiosum and occurs in both tropical and subtropical countries. Thalassemic patients, farmers, and aquatic-habitat residents are predisposed to this disease. Delayed treatment due to the long time required for isolation and identification of the causative organism, as well as the difficulty in obtaining internal organ specimens, results in high morbidity and mortality. To facilitate rapid diagnosis, an in-house enzyme-linked immunosorbent assay (ELISA) for the detection of immunoglobulin G antibodies against P. insidiosum was developed and evaluated for the diagnosis and monitoring of human pythiosis. Sixteen sera were collected from seven culture-proven human pythiosis cases. A total of 142 sera from thalassemic patients, from patients with other infectious diseases, and from healthy blood donors served as controls. All sera were tested in duplicate. By choosing a suitable cutoff point to maximize sensitivity and specificity, sera from pythiosis cases were all determined to be positive, whereas sera from control groups were all determined to be negative. ELISA signals from serial samples of sera taken from treated patients showed gradually declining levels of antibodies to P. insidiosum. The ELISA test was highly sensitive (100%) and specific (100%) and was useful for early diagnosis and for monitoring the treatment for pythiosis.
机译：人化脓毒症是由 Pythium insidiosum 引起的一种新兴的致命性传染病，在热带和亚热带国家均发生。地中海贫血患者，农民和水生动物居民易患此病。由于分离和鉴定致病生物所需的时间长，以及由于难以获得内脏器官标本而导致的延误治疗会导致较高的发病率和死亡率。为了促进快速诊断，一种内部酶联免疫吸附测定（ELISA）用于检测针对 P的免疫球蛋白G抗体。开发并评估了Insidiosum ，用于诊断和监测人的烟毒症。从7例经文化证明的人化脓毒症病例中收集了16份血清。来自地中海贫血患者，患有其他传染病的患者和健康献血者的总共142份血清作为对照。所有血清一式两份进行测试。通过选择合适的临界点以最大程度地提高敏感性和特异性，可以确定化脓性脓毒症病例的血清均为阳性，而对照组的血清则均为阴性。来自治疗患者的血清系列样品的ELISA信号显示 P抗体水平逐渐下降。惯用。 ELISA检测具有很高的敏感性（100％）和特异性（100％），可用于早期诊断和监测烟毒症的治疗。

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