Restriction fragment length polymorphism of the periplasmic flagellar flaA1 gene of Serpulina species.

L N Fisher; M R Mathiesen; G E Duhamel

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Restriction fragment length polymorphism of the periplasmic flagellar flaA1 gene of Serpulina species.

机译：Serpulina种的周质鞭毛flaA1基因的限制性片段长度多态性。

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Forty-one reference and field isolates of intestinal spirochetes representing Serpulina hyodysenteriae, Serpulina innocens, Serpulina pilosicoli, Brachyspira aalborgi, and nonclassified weakly beta-hemolytic intestinal spirochetes were compared by restriction fragment length polymorphism (RFLP) of the periplasmic flagellar (PF) flaA1 gene. Six genetically distinct groups (I through VI), each with a unique RFLP fingerprint pattern, were identified by Southern blotting analysis of EcoRV chromosomal DNA digests with a PCR-amplified digoxigenin-labeled 1-kb fragment of the S. hyodysenteriae isolate B78 PF flaA1 gene. The RFLP fingerprint patterns corresponded to known DNA homology differences between Serpulina species and to provisionally designated species described previously by using phenotypic and genotypic classification schemes. RFLP fingerprinting of the PF flaA1 gene provides a relatively simple genotypic method for identification of intestinal spirochetes without the use of radioisotopes.

机译：通过周质鞭毛1PF（RFLP）的限制性片段长度多态性（RFLP）比较了代表螺旋体猪痢疾螺旋体，无性Serpulina innocens，无药性Serpulina pilosicoli，短螺旋体aalborgi和未分类的弱β-溶血性肠螺旋菌的41种参考和野外分离株。。六个遗传学上不同的组（I至VI），每个组具有独特的RFLP指纹图谱，通过PCR扩增的地高辛耶尔森氏菌B78 PF flaA1的地高辛配基标记的1-kb片段对EcoRV染色体DNA消化液进行了Southern印迹分析，从而确定了每个组基因。 RFLP指纹图谱对应于Serpulina物种之间的已知DNA同源性差异，以及对应于先前通过使用表型和基因型分类方案描述的临时指定物种。 PF flaA1基因的RFLP指纹图谱提供了一种相对简单的基因型方法，可在不使用放射性同位素的情况下鉴定肠螺旋体。

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《Clinical and diagnostic laboratory immunology》 |1997年第6期|共6页
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L N Fisher; M R Mathiesen; G E Duhamel;
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1. Immunoblot reactivity of polyclonal and monoclonal antibodies with periplasmic flagellar proteins FlaA1 and FlaB of porcine Serpulina species. [J] . L N Fisher, G E Duhamel, R B Westerman, Clinical and diagnostic laboratory immunology . 1997,第4期

机译：多克隆和单克隆抗体与猪Serpulina物种的周质鞭毛蛋白FlaA1和FlaB的免疫印迹反应性。
2. Inactivation of Serpulina hyodysenteriae flaA1 and flaB1 periplasmic flagellar genes by electroporation-mediated allelic exchange. [J] . E L Rosey, M J Kennedy, D K Petrella, Journal of bacteriology . 1995,第20期

机译：通过电穿孔介导的等位基因交换灭活猪痢疾螺旋体flaA1和flaB1周质鞭毛基因。
3. Mapping flagellar genes in Chlamydomonas using restriction fragment length polymorphisms. [J] . C D Silflow, J A Schloss, L P Ranum, Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation . 1988,第1期

机译：使用限制片段长度多态性定位衣藻鞭毛基因。
4. IDENTIFICATION AND TYPING OF PORCINE INTESTINAL SPIROCHETES BY PCR-RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS OF THE nox GENE [C] . D.E.S.N. Barcellos, N. Ikuta, V.R. Lunge, Congress of the International Pig Veterinary Society . 2000

机译：通过PCR限制性片段长度多态性分析NOx基因鉴定和鉴定猪肠螺旋细胞
5. Restriction fragment length polymorphism and sequence analysis of ribosomal DNA for mapping and phylogenetic inference of Lentinula species. [D] . Nicholson, Michael Steven. 1995

机译：限制性片段长度多态性和核糖体DNA序列分析，用于香菇菌种的作图和系统发育推断。
6. Restriction fragment length polymorphism of the periplasmic flagellar flaA1 gene of Serpulina species. [O] . L N Fisher, M R Mathiesen, G E Duhamel 1997

机译：Serpulina种的周质鞭毛flaA1基因的限制性片段长度多态性。
7. Restriction fragment length polymorphism of the periplasmic flagellar flaA1 gene of Serpulina species [O] . L N Fisher, M R Mathiesen, G E Duhamel 1997

机译：丝网物种周质鞭毛FLAA1基因的限制性片段长度多态性

Restriction fragment length polymorphism of the periplasmic flagellar flaA1 gene of Serpulina species.

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