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首页> 外文会议>Congress of the International Pig Veterinary Society >IDENTIFICATION AND TYPING OF PORCINE INTESTINAL SPIROCHETES BY PCR-RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS OF THE nox GENE
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IDENTIFICATION AND TYPING OF PORCINE INTESTINAL SPIROCHETES BY PCR-RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS OF THE nox GENE

机译：通过PCR限制性片段长度多态性分析NOx基因鉴定和鉴定猪肠螺旋细胞

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摘要

The genus Serpulina includes certain pathogenic intestinal spirochetes and some species of the genus have recently been unified under the genus designation of Brachyspira (B.). B. hyodysenteriae is the agent of swine dysentery (3) and Serpulina (S.) intermedia has been implicated as a cause of typhilitis and colitis in poultry. B. alyinipulli is a cause of diarrhea in chickens and B. pilosicoli is the agent of intestinal spirochaetosis in pigs and also infects a number of other species, including humans, poultry and dogs. B. innocens and B. murdochii are considered nonpathogenic commensal organisms (3). Identification of spirochetes was originally performed on the basis of phenotypic methods (2,3). More recently, a variety of genetic-based methods have been developed, including amplification of DNA sequences by polymerase chain reaction (PCR) assays of sequences of 16S rRNA, 23S rRNA and the nox gene (1,2). These techniques are very useful for typing purposes, but individual amplifications must becarried out to diagnose each spirochetal species. The present study examined reference isolates of swine intestinal spirochetes representing previously established genetic groups using RFLP of a 307-bp sequence of the nox gene obtained by PCR amplification with conserved primers.
机译：属蛇形包括某些致病性肠道螺旋体和属的一些种类最近已短螺旋体（B.）的属指定之下统一。猪痢疾短螺旋是猪痢疾（3）和蛇形（S.）中间的代理已被暗示为typhilitis的原因，并且在家禽结肠炎。 B. alyinipulli是腹泻鸡的原因和B pilosicoli是在猪肠道螺旋体病的药剂，也感染了一些其他物种，包括人类，家禽和狗。 B. innocens和B. murdochii被认为是非致病性的共生生物（3）。最初的表型的方法（2,3）的基础上进行的螺旋体的鉴定。最近，各种各样的基于基因方法已经被开发，包括的16S rRNA，23S rRNA和NOx的基因（1,2）的序列的聚合酶链式反应（PCR）测定DNA序列的扩增。这些技术对于打字非常有用的，但个别扩增必须becarried出用于诊断每个螺旋体物种。本研究中检查表示使用通过PCR扩增与引物保守获得所述NOx基因的307-bp的序列的RFLP先前建立的遗传组猪肠道螺旋体的参考菌株。

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