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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Detection of HLA class I-specific antibodies by the QuikScreen enzyme-linked immunosorbent assay.
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Detection of HLA class I-specific antibodies by the QuikScreen enzyme-linked immunosorbent assay.

机译：通过QuikScreen酶联免疫吸附测定法检测HLA I类特异性抗体。

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The GTI QuikScreen test is an enzyme-linked immunosorbent assay (ELISA) that uses soluble HLA class I antigens as targets. In tests of 5,893 human serum specimens, we evaluated the reliability, sensitivity, and utility of the GTI QuikScreen test for detecting HLA class I-specific antibody. We found that the test could reliably detect HLA-specific antibodies of the immunoglobulin G (IgG) but not the IgM class. The degree of correlation with lymphocytotoxicity testing varied among the different serum sources, with the best correlation achieved with sera from renal transplant candidates (r > 0.7) and the poorest with sera from patients with end-stage liver disease (r = 0.26), possibly because of elevated alkaline phosphatase levels in the liver patients. Test reproducibility was high (96%), and test failure rate was low (1.7%). The test sensitivity is comparable to that of the antiglobulin cytotoxicity and, possibly, even flow cytometric tests. There was a highly significant (P < 0.001) correlation between the optical densities obtained in the ELISA and the percent panel reactive antibody determined by cytotoxicity testing. Therefore, although designed only to determine the presence or absence of HLA-specific antibody, GTI QuikScreen test results also provided an indication of the extent of sensitization. The test is one of the most effective and efficient ways to determine if antibodies producing a positive result in crossmatch tests are specific for HLA class I antigens. As an adjunct to serum screening by cytotoxicity testing, the GTI QuikScreen test can produce a substantial savings of time and effort that reduces the cost to the laboratory and to the patient.
机译：GTI QuikScreen测试是一种酶联免疫吸附测定（ELISA），使用可溶性HLA I类抗原作为靶标。在对5,893名人类血清样本的测试中，我们评估了GTI QuikScreen测试用于检测HLA I类特异性抗体的可靠性，敏感性和实用性。我们发现该测试可以可靠地检测免疫球蛋白G（IgG）的HLA特异性抗体，但不能检测IgM类。在不同血清来源中，与淋巴细胞毒性测试的相关程度各不相同，与肾脏移植候选者血清的最佳相关性（r> 0.7），与晚期肝病患者血清的最弱相关性（r = 0.26）因为肝脏患者碱性磷酸酶水平升高。测试重现性高（96％），测试失败率低（1.7％）。该测试的灵敏度与抗球蛋白的细胞毒性乃至流式细胞仪测试相当。 ELISA中获得的光密度与通过细胞毒性测试确定的面板反应性抗体百分率之间存在极显着的相关性（P <0.001）。因此，尽管仅用于确定HLA特异性抗体的存在或存在，但GTI QuikScreen测试结果也提供了致敏程度的指标。该测试是确定在交叉匹配测试中产生阳性结果的抗体是否特异性针对HLA I类抗原的最有效和有效的方法之一。作为通过细胞毒性测试进行血清筛选的辅助手段，GTI QuikScreen测试可以节省大量时间和精力，从而降低了实验室和患者的成本。

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