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Geometrical ordering of DNA in bacteria

机译:细菌中DNA的几何顺序

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The bacterium Caulobacter crescentus shows a remarkable spatial ordering of its chromosome that leads to a strong linear correlation between the position of genes on the chromosomal map and their spatial position in the cellular volume. In a recent study we have shown that a robust and universal geometrical ordering mechanism can explain this correlation. We demonstrated that self-avoidance of DNA, specific positioning of one or few DNA loci (such as origin or terminus) together with the action of DNA compaction proteins (that organize the chromosome into topological domains) are sufficient to get a linear arrangement of the chromosome along the cell axis. This configuration, however, only represents the population average. Individual cells can have DNA arrangements that deviate significantly from the mean configuration and that break left-right symmetry. Symmetry breaking is stronger for longer chromosomes.
机译:新月形细菌显示其染色体显着的空间有序性,从而导致染色体图谱上的基因位置与其在细胞体积中的空间位置之间具有很强的线性相关性。在最近的研究中,我们已经显示出健壮且通用的几何排序机制可以解释这种相关性。我们证明了DNA的自我回避,一个或几个DNA位点(例如起源或末端)的特异性定位以及DNA紧缩蛋白(将染色体组织成拓扑结构域)的作用足以获得线性排列。沿细胞轴的染色体。但是,此配置仅代表总体平均值。单个细胞可以具有明显偏离平均构型并破坏左右对称性的DNA排列。对于更长的染色体,对称性断裂更强。

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