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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Development of Monoclonal Antibodies againstUreaplasma urealyticum Serotypes and Their Use for Serotyping Clinical Isolates
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Development of Monoclonal Antibodies againstUreaplasma urealyticum Serotypes and Their Use for Serotyping Clinical Isolates

机译：抗解脲脲原体血清型单克隆抗体的开发及其在临床分离株血清分型中的应用

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Monoclonal antibodies (MAbs) against Ureaplasma urealyticum serotype 2, 5, 7, 8, 10, 11, 12, and 13 reference strains were developed. The reactivities of these MAbs with the 14 serotype reference strains was verified by colony immunofluorescence assay and Western blot assay. MAbs against serotypes 2, 7, 10, 11, and 12 were serotype specific, whereas MAbs against serotypes 5, 8, and 13 showed cross-reactivity. All MAbs against serotype 5 were cross-reactive with serotype 2, and one showed, in addition, cross-reactivity to serotypes 9 and 10. Mutual cross-reactivities were observed between MAbs against serotypes 8 and 13. The usefulness of the MAbs for the serotyping of U. urealyticum strains was evaluated by serotyping 21 selected clinical isolates. A complete set of MAbs (the newly developed MAbs and the previously described MAbs against serotypes 1, 3, 4, 6, 9, and 14) as well as a complete set of polyclonal antibodies (PAbs), PAbs 1 to 14, were used. MAbs were able to identify 18 of 21 isolates including 2 isolates with mixed serotypes. Polyreactivity, which occurred with 19 of the 21 isolates with PAbs, was not observed by the use of MAbs. MAbs seem to be a more valuable tool than PAbs for serotyping and could help in investigating a possible link between the expression or variability of the serotype-specific antigens and pathogenicity.
机译：研发了针对解脲支原体血清型2、5、7、8、10、11、12和13个参考菌株的单克隆抗体（MAb）。这些单克隆抗体与14种血清型参考菌株的反应性通过菌落免疫荧光法和Western印迹法验证。抗血清型2、7、10、11和12的单克隆抗体具有血清型特异性，而抗血清型5、8和13的单克隆抗体表现出交叉反应性。所有抗血清型5的单克隆抗体都与血清型2发生交叉反应，此外，一个抗体还显示与血清型9和10发生交叉反应。 U的血清分型。通过对21种选定的临床分离株进行血清分型来评估解脲支原体菌株。使用了一套完整的单克隆抗体（新开发的针对血清型1、3、4、6、9和14的单克隆抗体和先前描述的单克隆抗体）以及一套完整的多克隆抗体（单克隆抗体）PAb 1至14。 。 MAb能够鉴定出21种分离株中的18种，包括2种具有混合血清型的分离株。使用单克隆抗体未观察到21种PAb分离株中的19种发生多反应性。对于血清分型而言，MAb似乎比PAbs更有价值，并且可以帮助调查血清型特异性抗原的表达或变异性与致病性之间的可能联系。

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