目的 了解性病门诊患者解脲脲原体(Uu)分离株的血清分型与分子分群的分布状况与相互关系.方法 采用液体加固体的方法从临床标本中分离获得解脲脲原体临床菌株,纯化后采用PCR扩增Uu多条带基因进行分子分群,采用代谢抑制试验进行血清分型.结果 从377例性病门诊患者中分离到Uu 117例,阳性率31%.其中男性54例,阳性率46%;女性63例,阳性率54%.成功进行分子分群114例,其中生物1群47例,占41.2%;生物2群67例,占58.8%;进行血清分型60例,其中单个型别41例,占68.3%;多型别合并19例,占31.7%.出现频率高的型别为7型24例(29.63%),2型19例(23.46%),14型11例(13.58%)和4型7例(8.64%).未见3、5、12、13 4种血清型别.16.7%Uu分离株(10/60)存在分型与分群结果不符情况.结论 性病门诊患者Uu分离株中,生物2群所占比例多于生物1群,血清分型以7、2、14、4等型别常见,发现部分菌株分子分群与血清分型结果不符现象.%Objective To investigate the correlation and distribution of biovar and serotype of Ureaplasma urealyticum(Uu) isolates in out-patients with sexually transmitted disease(STD). Methods The liquid culture and solid culture were applied to isolating Uu strains from the clinical specimens. Af-ter purification of Uu strains, the multiple banded antigen gene of Uu was amplified by using polymer-ase chain reaction(PCR),and then the biovar of Uu isolates was performed. The serotype was deter-mined with metabolic inhibition test. Results Totally 117 cases(31%) were Uu positive from 377 clin-ical specimens of STD out-patients,Among them,54 cases(46%) were isolated from males,and 63 ca-ses(54%) were from females. A total of 114 isolates were successfully biovared,in which 47 cases (41.2%) were biovar 1 ,and 67 cases(58. 8%) were biovar 2;60 isolates were serotyped,in which 41 cases(68.3%) were single serotype,and 19 cases(31.7%) contained multiple serotypes. The most fre-quent serotypes were as follows: serotype 7 (24 cases, 29.63 %);serotype 2 (19 cases, 23.46%);sero-type 14(11 cases,13. 58%);serotype 4(7 cases,8.64%).No serotype 3,5,12,or 13 was detected. The discrepancy between biovar and serotype was found in 16. 7%(10/60) of Uu isolates. Conclusion The main biovar of Uu isolates is biovar 1 in STD out-patients,and serotype 7,2,14 and 4 were dominant. The discrepancy between biovar and serotype exists in some Uu isolates.
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