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Opsonization and Phagocytosis of Plasmodium falciparum Merozoites Measured by Flow Cytometry

机译：流式细胞仪测定恶性疟原虫裂殖子的调理作用和吞噬作用

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A flow cytometric phagocytosis assay was established to investigate the role of anti-merozoite antibody, complement, and cytokines on the phagocytosis of Plasmodium falciparum merozoites by human neutrophils. This assay involved allowing fluorescein isothiocyanate-labeled merozoites to interact with phagocytes and analysis of the cells on a FACScan with Lysis II software. To differentiate the proportion of neutrophil surface-bound merozoites from the merozoites ingested by neutrophils, the fluorescence of bound merozoites was quenched by adding trypan blue. The data showed that sera from malaria-immune individuals in the Solomon Islands and Papua New Guinea promoted merozoite engulfment by neutrophils. The cytokines tumor necrosis factor alpha, gamma interferon, granulocyte-macrophage colony-stimulating factor, and interleukin-1β significantly increased the amount and the rate of merozoite phagocytosis by neutrophils. Optimum merozoite phagocytosis occurred when both cytokines and anti-malarial antibody were present.
机译：建立了流式细胞仪吞噬作用试验，以研究抗裂殖子抗体，补体和细胞因子在人嗜中性粒细胞吞噬恶性疟原虫裂殖子中的作用。该测定涉及允许荧光素异硫氰酸酯标记的裂殖子与吞噬细胞相互作用，并使用Lysis II软件在FACScan上分析细胞。为了区分嗜中性粒细胞表面结合的裂殖子与嗜中性粒细胞摄取的裂殖子的比例，通过加入锥虫蓝淬灭结合的裂殖子的荧光。数据显示，所罗门群岛和巴布亚新几内亚的具有疟疾免疫力的人的血清促进了中性粒细胞吞噬裂殖子。细胞因子肿瘤坏死因子α，γ干扰素，粒细胞巨噬细胞集落刺激因子和白细胞介素1β显着增加了中性粒细胞吞噬裂殖子的数量和发生率。当同时存在细胞因子和抗疟疾抗体时，最佳裂殖子发生吞噬作用。

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