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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Antibody Responses in Patients with Staphylococcal Septicemia against Two Staphylococcus aureus Fibrinogen Binding Proteins: Clumping Factor and an Extracellular Fibrinogen Binding Protein
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Antibody Responses in Patients with Staphylococcal Septicemia against Two Staphylococcus aureus Fibrinogen Binding Proteins: Clumping Factor and an Extracellular Fibrinogen Binding Protein

机译：葡萄球菌败血症患者对两种金黄色葡萄球菌纤维蛋白原结合蛋白的抗体应答：聚集因子和细胞外纤维蛋白原结合蛋白

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We analyzed the serum antibody responses against twoStaphylococcus aureus fibrinogen binding proteins, the cell-bound clumping factor (Clf) and an extracellular fibrinogen binding protein (Efb). The material consisted of 105 consecutive serum samples from 41 patients suffering from S. aureussepticemia and 72 serum samples from healthy individuals. An enzyme-linked immunosorbent assay (ELISA) was developed. Healthy individuals showed variable levels of antibodies against the studied antigens, and cutoff levels (upper 95th percentile) against these antigens were determined. No correlation was seen between serum antibody levels against Clf and Efb. In acute-phase samples 27% of patients showed positive antibody levels against Clf and 10% showed positive levels against Efb, while in convalescent-phase samples 63% (26 of 41) showed a positive serology against Clf and 49% (20 of 41) showed a positive serology against Efb. Antibody levels against Efb were significantly lower in the acute-phase sera than in sera from healthy individuals (P = 0.002). An antibody response against Clf was most frequent in patients suffering from osteitis plus septic arthritis and from endocarditis (80% positive). The antibody response against Efb appeared to develop later in the course of disease. A possible biological effect of measured antibodies was demonstrated with the help of an inhibition ELISA, in which both high-titer and low-titer sera inhibited the binding of bacteria to fibrinogen. In conclusion, we have demonstrated in vivo production ofS. aureus fibrinogen binding proteins during deep S. aureus infections and a possible diagnostic and prophylactic role of the corresponding serum antibodies in such infections.
机译：我们分析了针对两种金黄色葡萄球菌纤维蛋白原结合蛋白，细胞结合聚集因子（Clf）和细胞外纤维蛋白原结合蛋白（Efb）的血清抗体反应。该材料由来自41名患有 S的患者的105个连续血清样本组成。金黄色葡萄球菌败血症和健康个体的72个血清样本。开发了一种酶联免疫吸附测定（ELISA）。健康个体显示出针对所研究抗原的可变抗体水平，并确定了针对这些抗原的临界水平（最高95％）。针对Clf和Efb的血清抗体水平之间没有相关性。在急性期样本中，有27％的患者针对Clf的抗体水平呈阳性，而10％的患者对Efb的抗体呈阳性水平，而在恢复期的样本中，有63％（41人中的26名）对Clf的血清学呈阳性，而49％（41人中的20名） ）显示出针对Efb的阳性血清学。急性期血清中针对Efb的抗体水平显着低于健康个体血清中（ P = 0.002）。在患有骨炎加化脓性关节炎和心内膜炎的患者中，最常见的抗Clf抗体反应（阳性率为80％）。针对Efb的抗体应答似乎在疾病过程中发展。借助于抑制ELISA证明了所测抗体的可能的生物学作用，其中高滴度和低滴度血清均抑制细菌与纤维蛋白原的结合。总之，我们已经证明了在体内产生em。 深部金黄色葡萄球菌纤维蛋白原结合蛋白金黄色葡萄球菌感染以及相应血清抗体在此类感染中的可能诊断和预防作用。

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