• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Fibrinogen-binding protein/clumping factor from Staphylococcus aureus.
【24h】

Fibrinogen-binding protein/clumping factor from Staphylococcus aureus.

机译：金黄色葡萄球菌的纤维蛋白原结合蛋白/聚集因子。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The binding of staphylococcal components to fibrinogen was studied. Fibrinogen-binding material from lysed staphylococcal cells or culture supernatants was affinity purified on fibrinogen-Sepharose and analyzed on Western (immuno-) blots by the use of fibrinogen and antifibrinogen antibodies. Two main bands of 87 and 19 kilodaltons (kDa) and a weaker band of 35 kDa bound specifically to fibrinogen. A monoclonal antibody bound to all three bands, indicating that these were of the same origin. The yield of these components was much higher in the culture supernatant than on washed cells, suggesting that these molecules are essentially extracellular products. In a plasma coagulase test, the 87-kDa band, but not the 19-kDa band, clotted rabbit plasma, demonstrating that the 87-kDa molecule is coagulase. This was further confirmed by the fact that the 87-kDa band binds specifically to prothrombin. It was shown that the 87- and the 19-kDa molecules were present on the cell surface by surface labeling the cells with 125I. In addition, the fact that killed and washed cells could induce plasma clotting demonstrates that staphylococci have coagulase exposed on the surface. It was concluded that cell-bound coagulase has affinity for fibrinogen also in the absence of prothrombin and thus is responsible for the clumping of staphylococci in fibrinogen.
机译：研究了葡萄球菌成分与纤维蛋白原的结合。将裂解的葡萄球菌细胞或培养物上清液中的纤维蛋白原结合材料在纤维蛋白原-琼脂糖上亲和纯化，并通过使用纤维蛋白原和抗纤维蛋白原抗体在Western（免疫）印迹上进行分析。两条主要带分别为87和19千道尔顿（kDa）和一条较弱的35 kDa带，专门结合到纤维蛋白原上。单克隆抗体结合所有三个条带，表明它们具有相同的来源。这些成分在培养上清液中的产率比在洗涤的细胞上的产率高得多，表明这些分子基本上是细胞外产物。在血浆凝结酶测试中，凝结的兔血浆凝结了87 kDa条带，但没有凝结19 kDa条带，表明87 kDa分子是凝结酶。 87-kDa条带与凝血酶原特异性结合的事实进一步证实了这一点。通过用125I对细胞进行表面标记，表明87-和19-kDa分子存在于细胞表面。另外，杀死和洗涤的细胞可以诱导血浆凝结的事实表明，葡萄球菌的表面上暴露有凝固酶。结论是，在没有凝血酶原的情况下，细胞结合的凝固酶也对血纤蛋白原具有亲和力，因此导致葡萄球菌在血纤蛋白原中结块。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号