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Serotype-Cross-Reactive Immunoglobulin M Responses in Dengue Virus Infections Determined by Enzyme-Linked Immunosorbent Assay

机译:通过酶联免疫吸附测定确定的登革热病毒感染中的血清型交叉反应性免疫球蛋白M反应

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We developed immunoglobulin M (IgM) antibody capture enzyme-linked immunosorbent assays (ELISAs) with four monovalent dengue virus antigens. We attempted to determine whether IgM responses in dengue virus infections are serotype specific or serotype cross-reactive. Serum samples from 14 confirmed dengue cases were examined. In these 14 cases, which consisted of 12 Japanese and 2 non-Japanese patients, infecting dengue virus serotypes were defined by reverse transcription-PCR. Thirteen of the 14 cases were IgM positive in ELISA. IgM responses were serotype cross-reactive in these 13 cases but were highest against infecting dengue virus serotype in 9 of the 13 cases. These results indicate that IgM responses are generally dengue serotype cross-reactive but that IgM levels are highest against the infecting serotype in most dengue cases.
机译:我们开发了具有四个单价登革热病毒抗原的免疫球蛋白M(IgM)抗体捕获酶联免疫吸附测定(ELISA)。我们试图确定登革热病毒感染中的IgM反应是血清型特异性还是血清型交叉反应。检查了14例确诊的登革热病例的血清样本。在这14例病例中,由12名日本人和2名非日本人患者组成,通过逆转录-PCR确定了感染登革病毒的血清型。 14例中有13例ELISA中IgM阳性。在这13例病例中,IgM反应具有血清型交叉反应性,但在13例中的9例中,抗感染登革热病毒血清型的IgM反应最高。这些结果表明,IgM反应通常是与登革热血清型交叉反应的,但是在大多数登革热病例中,IgM水平对感染的血清型最高。

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