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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Comparison of PanBio Dengue Duo Enzyme-Linked Immunosorbent Assay (ELISA) and MRL Dengue Fever Virus Immunoglobulin M Capture ELISA for Diagnosis of Dengue Virus Infections in Southeast Asia
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Comparison of PanBio Dengue Duo Enzyme-Linked Immunosorbent Assay (ELISA) and MRL Dengue Fever Virus Immunoglobulin M Capture ELISA for Diagnosis of Dengue Virus Infections in Southeast Asia

机译：PanBio登革热双核酶联免疫吸附测定（ELISA）与MRL登革热病毒免疫球蛋白M捕获ELISA在东南亚登革热病毒感染诊断中的比较

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The performances of the MRL dengue fever virus immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay (ELISA) and the PanBio Dengue Duo IgM capture and IgG capture ELISA were compared. Eighty sera from patients with dengue virus infections, 24 sera from patients with Japanese encephalitis (JE), and 78 sera from patients with nonflavivirus infections, such as malaria, typhoid, leptospirosis, and scrub typhus, were used. The MRL test showed superior sensitivity for dengue virus infections (94 versus 89%), while the PanBio test showed superior specificity for JE (79 versus 25%) and other infections (100 versus 91%). The PanBio ELISA showed better overall performance, as assessed by the sum of sensitivity and specificity (F value). When dengue virus and nonflavivirus infections were compared, F values of 189 and 185 were obtained for the PanBio and MRL tests, respectively, while when dengue virus infections and JE were compared, F values of 168 and 119 were obtained. The results obtained with individual sera in the PanBio and MRL IgM ELISAs showed good correlation, but this analysis revealed that the cutoff value of the MRL test was set well below that of the PanBio test. Comparing the sensitivity and specificity of the tests at different cutoff values (receiver-operator analysis) revealed that the MRL and PanBio IgM ELISAs performed similarly in distinguishing dengue virus from nonflavivirus infections, although the PanBio IgM ELISA showed significantly better distinction between dengue virus infections and JE. The implications of these findings for the laboratory diagnosis of dengue are discussed.
机译：比较了MRL登革热病毒免疫球蛋白M（IgM）捕获酶联免疫吸附测定（ELISA）和PanBio Dengue Duo IgM捕获和IgG捕获ELISA的性能。使用了80例登革病毒感染患者的血清，24例日本脑炎（JE）患者的血清和78例非黄病毒感染患者（如疟疾，伤寒，钩端螺旋体病和灌木斑疹伤寒）的血清。 MRL测试显示对登革热病毒感染具有更高的敏感性（94对89％），而PanBio测试显示对JE和其他感染（100对91％）具有更高的特异性。通过敏感性和特异性（ F 值）的总和评估，PanBio ELISA表现出更好的整体性能。比较登革热病毒和非黄病毒感染时，PanBio和MRL测试的 F 值分别为189和185，而比较登革热病毒感染和JE时， F >获得168和119的值。在PanBio和MRL IgM ELISA中使用单个血清获得的结果显示出良好的相关性，但是该分析表明MRL测试的临界值设置为远低于PanBio测试的临界值。在不同的临界值下比较测试的敏感性和特异性（接受者-操作者分析），尽管PanBio IgM ELISA显着更好地区分了登革热病毒感染和非结核分枝杆菌感染，但MRL和PanBio IgM ELISA在区分登革热病毒和非黄病毒方面表现相似。 JE。讨论了这些发现对登革热实验室诊断的意义。

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