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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Use of a Dry-Plasma Collection Device to Overcome Problems with Storage and Transportation of Blood Samples for Epidemiology Studies in Developing Countries
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Use of a Dry-Plasma Collection Device to Overcome Problems with Storage and Transportation of Blood Samples for Epidemiology Studies in Developing Countries

机译：使用干血浆采集装置克服血液样本的存储和运输问题，以进行发展中国家的流行病学研究

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Studies are difficult in areas lacking modern facilities due to the inability to reliably collect, store, and ship samples. Thus, we sought to evaluate the use of a dry plasma collection device for seroepidemiology studies. Plasma was obtained by fingerstick using a commercial dry plasma collection device (Chemcard Plasma Collection Device) and serum (venipuncture) from individuals in Kazakhstan. Plasma samples were air dried for 15 min and then stored desiccated in foil zip-lock pouches at 4 to 6°C and subsequently shipped to the United States by air at ambient temperature. Serum samples remained frozen at ?20°C until assayed. Helicobacter pylori status was determined by enzyme-linked immunosorbent assay (HM-CAP EIA) for the dry plasma and the serum samples. The results were concordant in 250 of the 289 cases (86.5%). In 25 cases (8.6%), the dry plasma samples gave indeterminate results and could not be retested because only one sample was collected. Five serum samples were positive, and the corresponding dry plasma samples were negative; one serum sample was negative, and the corresponding plasma sample was positive. The relative sensitivity and specificity of the Chemcard samples to serum were 97.6 and 97.9%, respectively, excluding those with indeterminate results. Repeated freeze-thawing had no adverse effect on the accuracy of the test. We found the dry plasma collection device to provide an accurate and practical alternative to serum when venipuncture may be difficult or inconvenient and sample storage and handling present difficulties, especially for seroepidemiologic studies in rural areas or developing countries and where freeze-thawing may be unavoidable.
机译：由于无法可靠地收集，存储和运送样品，在缺乏现代化设施的地区进行研究非常困难。因此，我们试图评估干血浆收集设备在血清流行病学研究中的应用。使用市售的干血浆收集装置（Chemcard Plasma Collection Device）通过指尖获得血浆，并从哈萨克斯坦的个体获得血清（静脉穿刺）。将血浆样品风干15分钟，然后将其干燥保存在4至6°C的铝箔自封袋中，然后在环境温度下通过空气运送到美国。血清样品保持在约20°C下冷冻直至测定。通过酶联免疫吸附法（HM-CAP EIA）测定干血浆和血清样品的幽门螺杆菌状态。 289例病例中有250例的结果一致（86.5％）。在25例（8.6％）的情况下，干燥血浆样品的结果不确定，由于仅收集了一个样品而无法重新测试。 5份血清样品为阳性，而相应的干血浆样品为阴性; 1个血清样品为阴性，而相应的血浆样品为阳性。 Chemcard样品对血清的相对敏感性和特异性分别为97.6％和97.9％，不包括不确定结果的样品。反复冻融对测试的准确性没有不利影响。我们发现，当静脉穿刺困难或不便且样品存储和处理存在困难时，尤其是对于农村地区或发展中国家的血清流行病学研究以及可能无法避免冻融的情况，干血浆收集装置可提供准确，实用的血清替代品。

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