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首页> 外文期刊>Journal of clinical laboratory analysis. >A New Blood Collection Device Minimizes Cellular DNA Release During Sample Storage and Shipping When Compared to a Standard Device
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A New Blood Collection Device Minimizes Cellular DNA Release During Sample Storage and Shipping When Compared to a Standard Device

机译：与标准设备相比，一种新的采血设备可最大程度地减少样品存储和运输过程中细胞DNA的释放

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Cell-free DNA (cfDNA) circulating in blood is currently used for noninvasive diagnostic and prognostic tests. Minimizing background DNA is vital for detection of low abundance cfDNA. We investigated whether a new blood collection device could reduce background levels of genomic DNA (gDNA) in plasma compared to KSUB3/SUBEDTA tubes, when subjected to conditions that may occur during sample storage and shipping.MethodsBlood samples were drawn from healthy donors into KSUB3/SUBEDTA and Cell-Free DNA? BCT (BCT). To simulate shipping, samples were shaken or left unshaken. In a shipping study, samples were shipped or not shipped. To assess temperature variations, samples were incubated at 6°C, 22°C, and 37°C. In all cases, plasma was harvested by centrifugation and total plasma DNA (pDNA) assayed by quantitative real-time polymerase chain reaction (qPCR).ResultsShaking and shipping blood in KSUB3/SUBEDTA tubes showed significant increases in pDNA, whereas no change was seen in BCTs. Blood in KSUB3/SUBEDTA tubes incubated at 6°C, 22°C, and 37°C showed increases in pDNA while pDNA from BCTs remained stable.ConclusionsBCTs prevent increases in gDNA levels that can occur during sample storage and shipping. This new device permits low abundance DNA target detection and allows accurate cfDNA concentrations.
机译：血液中循环的无细胞DNA（cfDNA）目前用于无创诊断和预后测试。最小化背景DNA对于检测低丰度cfDNA至关重要。我们调查了一种新的采血设备在样品存储和运输过程中可能遇到的条件下，与K 3 EDTA管相比是否能降低血浆中的基因组DNA（gDNA）的背景水平。从健康供体中提取到K 3 EDTA和无细胞DNA中？ BCT（BCT）。为了模拟运输，将样品摇动或摇动。在运输研究中，样品已运输或未运输。为了评估温度变化，将样品在6°C，22°C和37°C下孵育。在所有情况下，通过离心收集血浆，并通过实时定量聚合酶链反应（qPCR）测定血浆总DNA（pDNA）。结果在K 3 EDTA管中摇动和运输血液显示pDNA显着增加，而BCT中没有发现变化。在分别于6°C，22°C和37°C孵育的K 3 EDTA管中的血液显示pDNA升高，而来自BCT的pDNA保持稳定。结论BCT阻止了样品存储过程中可能发生的gDNA水平升高。和运输。这种新设备允许低丰度的DNA靶标检测并允许精确的cfDNA浓度。

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