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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Analysis of Complement Fixation and Commercial Enzyme Immunoassays for Detection of Antibodies to Mycoplasma pneumoniae in Human Serum
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Analysis of Complement Fixation and Commercial Enzyme Immunoassays for Detection of Antibodies to Mycoplasma pneumoniae in Human Serum

机译：补体固定和商业酶免疫分析法检测人血清中肺炎支原体抗体的分析

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The Meridian ImmunoCard (IC), GenBio ImmunoWELL-IgM, and Remel EIA commercial antibody tests are qualitative enzyme immunoassays that detect antibodies to Mycoplasma pneumoniae in serum. These tests were compared to an M. pneumoniae complement fixation (CF) assay, which uses a commercially available antigen component. The Meridian IC and the ImmunoWELL-IgM detect immunoglobulin M (IgM) only; the Remel EIA and the CF test detect both IgM and IgG antibodies. Detection of specific IgM antibody, which appears early in infection, can be, but is not always, indicative of a recent or current infection. Paired serum samples from 64 adult patients with probable M. pneumoniae infection were examined with each of the four tests. Thirty (47%) of the 64 acute-phase sera were IgM positive by Meridian IC, 26 (41%) were positive by Remel EIA, 24 (38%) were positive by CF, and 15 (23%) were positive by ImmunoWELL-IgM. When both the acute- and convalescent-phase serum samples from each patient were examined, 61 (95%) of the 64 patients were positive by CF, 60 patients (94%) were positive by Remel EIA, 52 patients (81%) were IgM positive by the Meridian IC, and 29 patients (45%) were IgM positive by the ImmunoWELL-IgM assay. The Meridian IC was more sensitive than the other tests for early detection of IgM antibodies. However, after examining paired serum samples, we concluded that the detection of IgM alone may not be useful for all cases of mycoplasma infection, especially in an adult population.
机译：Meridian ImmunoCard（IC），GenBio ImmunoWELL-IgM和Remel EIA商业抗体检测是定性酶免疫检测，可检测血清中肺炎支原体的抗体。将这些测试与 M进行比较。肺炎补体固定法（CF），使用市售的抗原成分。经络IC和ImmunoWELL-IgM仅检测免疫球蛋白M（IgM）; Remel EIA和CF测试可检测IgM和IgG抗体。在感染早期出现的特异性IgM抗体的检测可以但不总是指示最近或当前的感染。来自64位可能患有 M的成年患者的配对血清样本。四个测试中的每一个都检查了肺炎的感染。经络IC检测到的64急性期血清中有30（47％）IgM阳性，Remel EIA检测到26（41％）阳性，CF检测为24（38％）阳性，ImmunoWELL检测为15（23％）阳性-IgM。当检查每位患者的急性和恢复期血清样本时，CF阳性的64例患者中有61例（95％），Remel EIA阳性的有60例（94％），52例（81％）呈阳性。经络IC呈IgM阳性，ImmunoWELL-IgM分析显示29例患者（45％）为IgM阳性。对于早期检测IgM抗体，子午线IC比其他测试更为敏感。但是，在检查了配对的血清样本后，我们得出结论，单独检测IgM可能并不适用于所有支原体感染病例，尤其是在成年人群中。

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