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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Analysis of Eight Commercial Enzyme Immunoassay Tests for Detection of Antibodies to Mycoplasma pneumoniae in Human Serum
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Analysis of Eight Commercial Enzyme Immunoassay Tests for Detection of Antibodies to Mycoplasma pneumoniae in Human Serum

机译：用于检测人血清中肺炎支原体抗体的八种商业酶免疫法测试分析

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Mycoplasma pneumoniae is an important etiologic agent of primary atypical pneumonia in children and adults. The diagnosis of M. pneumoniae infection is commonly confirmed through serologic testing. In this study, we used paired sera from 51 patients (all with confirmed M. pneumoniae infection and positive complement fixation [CF] titers) to compare the results of eight enzyme immunoassays (EIAs) available commercially in the United States. We compared two single-use EIAs and six plate-type EIAs. Results from acute-phase sera ranged from only 7 (14%) positive by ImmunoWELL (GenBio) immunoglobulin M (IgM) EIA to 23 (45%) positive by Zeus IgG EIA. When both the acute-phase and convalescent-phase serum samples were examined, positive results ranged from 20 (39%) by the ImmunoWELL (GenBio) IgM assay to 45 (88%) positive by the Remel IgG-IgM EIA. In this study, the single-use EIAs by Remel and Meridian were more reliable than were the plate-type EIAs. Among the plate-type EIAs, the Zeus and DiaSorin assays (which detect antibodies to protein antigens) were more sensitive than the ImmunoWELL assay (which detects antibodies to glycolipid antigens). In general, IgG EIAs on convalescent-phase sera were more concordant with one another than were IgM EIAs with one another. Scatter plot analysis of convalescent-phase sera showed that, as the CF titer dropped, the IgM assays identified fewer positive convalescent-phase sera. In contrast, the IgG assays provided fairly consistent positive results for convalescent-phase sera with CF titers of 64 and above. Results of individual tests and overall limitations of serodiagnostics for M. pneumoniae infections are discussed.
机译：肺炎支原体是儿童和成人原发性非典型肺炎的重要病因。 M的诊断。肺炎感染通常通过血清学检查确定。在这项研究中，我们使用了51例患者的配对血清（均已确诊肺炎支原体感染且补体固定[CF]滴度为阳性），以比较八种商业上可用的酶免疫测定（EIA）的结果。美国。我们比较了两个一次性EIA和六个板式EIA。急性期血清的结果范围从ImmunoWELL（GenBio）免疫球蛋白M（IgM）EIA阳性仅7（14％）阳性到Zeus IgG EIA阳性23（45％）阳性。当同时检查急性期和恢复期血清样品时，阳性结果的范围从ImmunoWELL（GenBio）IgM测定的20（39％）到Remel IgG-IgM EIA的45（88％）阳性。在这项研究中，Remel和Meridian的一次性EIA比板式EIA更可靠。在平板型EIA中，Zeus和DiaSorin检测（检测抗蛋白质抗原的抗体）比ImmunoWELL检测（检测糖脂抗原的抗体）更灵敏。通常，恢复期血清中的IgG EIA比IgM EIA彼此更一致。恢复期血清的散点图分析表明，随着CF滴度的降低，IgM分析可识别出更少的阳性恢复期血清。相反，IgG滴度为CF滴度为64或更高的恢复期血清提供了相当一致的阳性结果。单个测试结果和 M血清学诊断的总体局限性。讨论了肺炎的感染。

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