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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Neutralization of Measles Virus Infectivity and Antibody-Dependent Cell-Mediated Cytotoxicity Activity against an Epstein-Barr Virus-Infected Cell Line by Intravenous Administration of Immunoglobulin G
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Neutralization of Measles Virus Infectivity and Antibody-Dependent Cell-Mediated Cytotoxicity Activity against an Epstein-Barr Virus-Infected Cell Line by Intravenous Administration of Immunoglobulin G

机译：通过静脉注射免疫球蛋白G来中和麻疹病毒感染性和抗体依赖性细胞介导的针对爱泼斯坦-巴尔病毒感染的细胞系的细胞毒活性

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摘要

Patients with antibody deficiency disorders are highly susceptible to microbial infections. Intravenous (i.v.) immunoglobulin concentrates were originally developed as replacement therapy for such patients. The present study assesses the measles virus neutralizing antibody titers and the antibody-dependent cell-mediated cytotoxicity (ADCC) capacities against Epstein-Barr virus (EBV)-infected cells of immunoglobulin G (IgG) preparations produced for i.v. use (i.v. IgG). The level of neutralizing antibodies against measles virus was determined by a syncytium neutralization test with Vero cells as targets. The measles virus neutralizing antibody titers of the i.v. IgG preparations were >3 × 102 and were an average of 1.0 log higher than the titers in pooled plasma from healthy subjects. The two IgG preparations tested showed similar ADCC activities against EBV-infected Raji cells, being active at concentrations of 3 mg/ml or higher. i.v. IgG bound to Raji cells but not to the EBV-negative Ramos cells, as evaluated by flow cytometry. Our in vitro findings may provide further support for the use of i.v. IgG for the prevention and treatment of infections caused by specific viral pathogens.
机译：患有抗体缺乏症的患者高度容易受到微生物感染。静脉（i.v.）免疫球蛋白浓缩物最初是作为此类患者的替代疗法而开发的。本研究评估了麻疹病毒中和抗体的效价和针对爱泼斯坦-巴尔病毒（EBV）感染的免疫球蛋白G（IgG）制剂制备的细胞的抗体依赖性细胞介导的细胞毒性（ADCC）能力。使用（静脉注射IgG）。通过以Vero细胞为靶标的合胞体中和测试确定针对麻疹病毒的中和抗体的水平。 i.v.的麻疹病毒中和抗体滴度IgG制剂> 3×10 2 ，比健康受试者的合并血浆中的滴度平均高1.0 log。所测试的两种IgG制剂对EBV感染的Raji细胞表现出相似的ADCC活性，在3 mg / ml或更高的浓度下具有活性。 i.v.通过流式细胞术评估，IgG与Raji细胞结合，但不与EBV阴性Ramos细胞结合。我们的体外研究结果可能会为i.v. IgG用于预防和治疗由特定病毒病原体引起的感染。

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