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首页> 外文期刊>Infection and immunity >Antibody-dependent cell-mediated cytotoxicity against varicella-zoster virus-infected targets.
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Antibody-dependent cell-mediated cytotoxicity against varicella-zoster virus-infected targets.

机译：对水痘带状疱疹病毒感染的靶标的抗体依赖性细胞介导的细胞毒性。

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摘要

Antibody-dependent cell-mediated cytotoxicity (ADCC) against cryopreserved varicella-zoster virus-infected human foreskin fibroblasts was detected in a 51Cr release assay. Target cells, samples of seropositive or seronegative sera, and mononuclear cells obtained by Ficoll-Hypaque centrifugation of human peripheral blood were added to microtiter plate wells and allowed to incubate at 37 degrees C for 4 h. Fibroblasts infected for 48 to 96 h were susceptible to ADCC. Effector cells from seropositive and seronegative normal children were equally active in the assay. Antibody titers were determined by testing serial dilutions of sera in the ADCC assay. Zoster immune globulin had a titer of 204,800. Sera from 40 naturally seropositive individuals were compared by assays for ADCC and fluorescent antibody to membrane antigen. All sera that were negative by fluorescent antibody to membrane antigen (less than 2) were also negative by ADCC (less than 20). All sera that were positive by fluorescent antibody to membrane antigen were also positive by ADCC, but titers of individual sera were frequently 5 to 20 times higher in the ADCC assay.
机译：在51Cr释放试验中检测到针对冷冻保存的水痘带状疱疹病毒感染的人包皮成纤维细胞的抗体依赖性细胞介导的细胞毒性（ADCC）。将目标细胞，血清阳性或血清阴性血清样品以及通过人外周血Ficoll-Hypaque离心获得的单核细胞添加到微量滴定板孔中，并在37°C下孵育4小时。感染48至96小时的成纤维细胞对ADCC敏感。来自血清阳性和血清阴性正常儿童的效应细胞在测定中具有同等活性。通过在ADCC分析中测试血清的系列稀释度来确定抗体效价。带状疱疹免疫球蛋白的滴度为204,800。通过测定ADCC和针对膜抗原的荧光抗体，比较了来自40位自然血清反应阳性个体的血清。所有抗膜抗原的荧光抗体阴性的血清（小于2）也被ADCC阴性（小于20）。所有抗膜抗原的荧光抗体呈阳性的血清均也呈ADCC阳性，但在ADCC分析中单个血清的滴度通常高5至20倍。

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