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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Contribution of Epitope Specificity to the Binding of Monoclonal Antibodies to the Capsule of Cryptococcus neoformans and the Soluble Form of Its Major Polysaccharide, Glucuronoxylomannan
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Contribution of Epitope Specificity to the Binding of Monoclonal Antibodies to the Capsule of Cryptococcus neoformans and the Soluble Form of Its Major Polysaccharide, Glucuronoxylomannan

机译：表位特异性对单克隆抗体与新型隐球菌及其主要多糖葡糖醛酸甘露聚糖的可溶形式的结合的贡献。

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Incubation of encapsulated cryptococci with monoclonal antibodies (MAbs) specific for glucuronoxylomannan (GXM), the major capsular polysaccharide of Cryptococcus neoformans, produces two distinct capsular quellung-type reactions termed rim and puffy. The type of capsular reaction that occurs is determined by the epitope specificity of the MAb and the serotype of the yeast cell. Several biological activities, including opsonic activity, complement activation, and protective efficacy, are associated with the type of capsular reaction produced by a MAb. The goal of this study was to examine the reactivities of two families of anti-GXM MAbs with serotype A and D capsular polysaccharides in several immunochemical assays, including agglutination, immunofluorescence, quantitative precipitation, and enzyme-linked immunosorbent assay, in an effort to identify serological assays that are predictive of the capsular quellung reaction. The results showed that the type of capsular reaction (rim versus puffy) is a qualitative assessment of antibody-capsule interaction that cannot be predicted on the basis of a serological assay. The results further showed that antibody reactivity demonstrated in one serological assay is not necessarily predictive of results in another assay, particularly in cases where one assay examines antibody-capsule interactions, e.g., agglutination, and another assay examines interaction of antibody with soluble GXM. Taken together, the results suggest caution in interpretation of immunochemical assays for anti-GXM antibodies and recommend the use of multiple assays formats when studying anticryptococcal antibodies.
机译：封装的隐球菌与特异葡糖新葡聚糖的主要荚膜多糖葡糖醛酸氧甘露聚糖（GXM）的单克隆抗体（MAb）一起孵育，会产生两种截然不同的荚膜囊化反应，称为边缘和浮肿。发生的荚膜反应的类型取决于MAb的表位特异性和酵母细胞的血清型。几种生物活性，包括调理活性，补体激活和保护功效，与MAb产生的荚膜反应类型有关。这项研究的目的是在凝集，免疫荧光，定量沉淀和酶联免疫吸附试验等几种免疫化学试验中，检查两个血清型A和D荚膜多糖的抗GXM MAb家族的反应性可以预测荚膜抑制反应的血清学检测。结果表明，荚膜反应的类型（边缘与浮肿）是抗体-胶囊相互作用的定性评估，无法根据血清学分析预测。结果进一步表明，在一种血清学测定法中证明的抗体反应性不一定预测另一种测定法的结果，特别是在其中一种测定法检查抗体-胶囊相互作用例如凝集，而另一种测定法检查抗体与可溶性GXM的相互作用的情况下。两者合计，结果建议在解释抗GXM抗体的免疫化学测定时要谨慎，并建议在研究抗隐球菌抗体时使用多种测定形式。

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