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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Antibody Responses to Escherichia coli O157 and Other Lipopolysaccharides in Healthy Children and Adults
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Antibody Responses to Escherichia coli O157 and Other Lipopolysaccharides in Healthy Children and Adults

机译：健康儿童和成人对大肠杆菌O157和其他脂多糖的抗体反应

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In Mexico, diarrheal disease due to different serotypes of Escherichia coli is highly prevalent, with only sporadic isolation of O157 non-H7 strains. This could be due to exposure to the O157 or related E. coli lipopolysaccharide (LPS), such as O7 or O116, at an early age. By using enzyme-linked immunosorbent assay (ELISA) and Western blotting, the present study analyzed 605 serum samples from Mexican adults and infants without clinical symptoms of disease for the presence of antibodies to these three E. coli LPSs. The bactericidal activities of homologous and heterologous rabbit and human serum samples against O7, O116, and O157 E. coli LPSs were also determined. By using a cutoff point of 0.7, it was found by the ELISAs that 28 of 562 (5%) of the serum samples from adolescents and adults and 2 of 43 (5%) of the serum samples from infants less than 1 year of age reacted with the O157 LPS. By using cutoff points between 0.4 and 0.699, the proportion of serum samples from both age groups that reacted with the O157 LPS increased to 20%. Western blotting analysis of selected serum samples that showed an intermediate response against the O157 LPS by the ELISAs showed that 61 of 88 (69%) reacted with the same LPS. A similar result was observed for maternal milk samples. The bactericidal activities of rabbit serum samples against the O7, O116, and O157 LPSs showed that they were positive for both homologous and heterologous antigens. Similar results were observed with the human serum samples. O157 non-H7 strains were identified in only 10% of the E. coli strains isolated from 263 Mexican children with and without diarrhea over the past 15 years. This absence of O157:H7 strains in Mexico may be associated with the presence of antibodies against O157 or related E. coli LPSs.
机译：在墨西哥，由于大肠杆菌的不同血清型引起的腹泻病非常普遍，仅偶尔分离出O157非H7菌株。这可能是由于暴露于O157或相关的 E。大肠菌脂多糖（LPS），例如O7或O116。通过使用酶联免疫吸附测定（ELISA）和Western印迹，本研究分析了来自墨西哥成人和无临床症状的婴儿的605份血清样品中是否存在针对这三种 E的抗体。大肠菌同源和异源兔和人血清样品对O7，O116和O157 E的杀菌活性。还确定了大肠埃希菌脂多糖。通过使用0.7的截断点，通过ELISA法发现，青少年和成人的562个样本中（28％）（5％）和1岁以下婴儿的43个样本中（5％）2个（5％）与O157 LPS反应。通过使用介于0.4和0.699之间的临界点，与O157 LPS反应的两个年龄组的血清样品的比例增加到20％。通过ELISA对选定的血清样品进行的Western印迹分析显示出对O157 LPS的中间反应，表明88个样品中有61个（69％）与相同的LPS反应。母乳样品也观察到类似结果。兔血清样品对O7，O116和O157 LPS的杀菌活性表明，它们对同源和异源抗原均呈阳性。用人血清样品观察到相似的结果。仅在 E 的10％中鉴定出O157非H7菌株。在过去15年中，从263名有腹泻和无腹泻的墨西哥儿童中分离出了 coli 菌株。墨西哥缺乏O157：H7菌株可能与抗O157抗体或相关的EmE有关。大肠菌

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