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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Humoral Immune Responses to S-Layer-Like Proteins of Bacteroides forsythus
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Humoral Immune Responses to S-Layer-Like Proteins of Bacteroides forsythus

机译：连翘细菌S层蛋白的体液免疫反应。

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Bacteroides forsythus is one of the important periodontopathic bacteria, and this microorganism is known to have an S-layer outside the outer membrane. The S-layer-like antigens were recently isolated from B. forsythus, and they were found to be 270- and 230-kDa proteins in the envelope fraction. In this study, these proteins were confirmed to be specific to B. forsythus by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and they were clearly recognized by sera from patients with adult and early-onset periodontitis in Western immmunoblot analysis. We compared the immunoglobulin G (IgG) responses against the purified S-layer-like antigen by enzyme-linked immunosorbent assay. IgG responses against this antigen were low in healthy control subjects, but they were significantly higher in subjects with adult and early-onset periodontitis. Together with the fact that the IgG responses against the crude extract of B. forsythus did not rise significantly in patients with periodontitis, S-layer-like proteins are considered to be specific antigens of B. forsythus and may play an important role in the progression of periodontitis.
机译：连翘杆菌是重要的牙周病细菌之一，并且已知该微生物在外膜外具有S层。最近从emB中分离出了S层样抗原。连翘，并且在包膜部分发现它们是270和230 kDa的蛋白质。在这项研究中，这些蛋白质被证实对 B具有特异性。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳对连翘进行鉴定，在西方免疫印迹分析中，它们已被成人和早发性牙周炎患者的血清清楚地识别。我们通过酶联免疫吸附试验比较了针对纯化的S层样抗原的免疫球蛋白G（IgG）反应。在健康对照组中，针对该抗原的IgG应答较低，但在成人和早发性牙周炎的受试者中，IgG应答明显较高。连同IgG对B的粗提物反应的事实。牙周炎患者的连翘没有明显升高，S层样蛋白被认为是 B的特异性抗原。连翘可能在牙周炎的进展中起重要作用。

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