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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Inflammatory Responses of Bovine Polymorphonuclear Neutrophils Induced by Staphylococcal Enterotoxin C via Stimulation of Mononuclear Cells
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Inflammatory Responses of Bovine Polymorphonuclear Neutrophils Induced by Staphylococcal Enterotoxin C via Stimulation of Mononuclear Cells

机译：葡萄球菌肠毒素C通过刺激单核细胞诱导的牛多形核中性粒细胞的炎症反应。

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To elucidate the pathological roles of staphylococcal enterotoxin C (SEC) in bovine staphylococcal mastitis, a histopathological analysis of SEC-inoculated mammary glands was performed. SEC-inoculated mammary glands exhibited interstitial inflammation, and the leukocytes that migrated into the gland were predominantly polymorphonuclear neutrophils (PMN). In the gland cistern tissues dissected from SEC-inoculated mammary glands, epithelial cellular degeneration was observed. We also investigated the physiological effects of SEC on PMN in vitro. PMN migration was induced by culture supernatant of SEC-stimulated peripheral blood mononuclear cells (S-PBMC sup) but not by that of nonstimulated PBMC (N-PBMC sup). The concentration of interleukin-8 was significantly (P < 0.05) higher in S-PBMC sup than N-PBMC sup, and a significantly (P < 0.05) higher mRNA expression of growth-regulated oncogenes was detected in SEC-stimulated PBMC than in nonstimulated PBMC. Milk PMN collected from SEC-inoculated mammary glands produced more than 2 times the amount of superoxide at 1 day postinoculation (dpi) than at 0 dpi in the presence of phorbol 12-myristate 13-acetate (PMA). PMN cultured with S-PBMC sup for 24 h also produced significantly (P < 0.05) larger amounts of superoxide than those cultured with N-PBMC sup in the presence of PMA. Moreover, S-PBMC sup induced the long-time survival of PMN. These results indicate that SEC induces the activation of PMN via the stimulation of mononuclear cells.
机译：为了阐明葡萄球菌肠毒素C（SEC）在牛葡萄球菌性乳腺炎中的病理作用，对SEC接种的乳腺进行了组织病理学分析。 SEC接种的乳腺表现出间质性炎症，迁移到该腺的白细胞主要是多形核中性粒细胞（PMN）。在从SEC接种的乳腺切开的腺水箱组织中，观察到上皮细胞变性。我们还研究了SEC对PMN的体外生理影响。 PMN迁移是由SEC刺激的外周血单个核细胞（S-PBMC sup）的培养上清液诱导的，而不是由未刺激的PBMC（N-PBMC sup）的培养上清液诱导的。 S-PBMC sup中白细胞介素8的浓度显着（ P <0.05）比N-PBMC sup高，并且mRNA表达显着（ P <0.05）在SEC刺激的PBMC中检测到生长调节的致癌基因的比例高于未刺激的PBMC。在存在佛波醇12-肉豆蔻酸酯13-醋酸酯（PMA）的情况下，从SEC接种的乳腺收集的牛奶PMN在接种后1天（dpi）产生的超氧化物量是在0 dpi时的2倍以上。在PMA存在下，用S-PBMC sup培养24 h的PMN产生的过氧化物也显着（ P <0.05）大得多。此外，S-PBMC sup诱导了PMN的长期存活。这些结果表明，SEC通过刺激单核细胞诱导了PMN的激活。

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