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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Detection of Anti-Leishmania Immunoglobulin G Antibodies in Urine Specimens of Dogs with Leishmaniasis
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Detection of Anti-Leishmania Immunoglobulin G Antibodies in Urine Specimens of Dogs with Leishmaniasis

机译：利什曼病犬尿液标本中抗利什曼原虫免疫球蛋白G抗体的检测

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For years, anti-Leishmania immunoglobulin G (IgG) antibodies have been detected in the sera of dogs living in areas of leishmaniasis endemicity. They have also been found in the aqueous humor and cerebrospinal fluid. In contrast, a review of the literature failed to identify the detection of anti-Leishmania antibodies in urine samples from dogs with leishmaniasis. Ninety-five dog urine samples were examined for the presence of anti-Leishmania antibodies by using a protein A enzyme-linked immunosorbent assay (ELISA). Twenty additional urine samples were collected from healthy dogs as controls. An IgG2 ELISA was performed on 26 urine samples found positive by the protein A ELISA. Twenty-three urine samples found positive to anti-Leishmania antibodies were tested for the local production of anti-Leishmania antibodies in the urinary tract by means of the urine antibody coefficient. Ten urine samples (and the corresponding serum samples) were compared by Western blot (WB) analysis. Thirty-five out of the 95 urine samples were found positive, 57 were found negative, and 3 were found inconclusive for antibody detection by the protein A ELISA. A high correlation between protein A and IgG2 levels was found in positive urine samples. Anti-Leishmania antibodies were present in the urine of dogs that had leishmaniasis, urinary protein/creatinine (U P/C) ratios of greater than one, and normal urinary sediment. A statistically significant correlation was observed between the U P/C ratios and the levels of anti-Leishmania antibodies in positive urine samples. In general, WB analysis and the urine antibody coefficient suggested that the presence of anti-Leishmania antibodies in urine was the consequence of an impairment of filtration of the glomerular barrier. However, in some dogs, WB analysis could be interpreted as suggesting that the presence of anti-Leishmania antibodies was caused, to a lesser extent, by local antibody production in the urinary tract. Antibody detection in urine could be a noninvasive method for leishmaniasis diagnosis and prognosis in dogs with glomerulonephropathies.
机译：多年来，在生活在利什曼病流行地区的狗的血清中已检测到抗 Leishmania 免疫球蛋白G（IgG）抗体。在房水和脑脊液中也发现了它们。相反，文献综述未能鉴定出在患有利什曼病的狗的尿液样品中检测到抗利什曼病抗体。通过使用蛋白A酶联免疫吸附测定（ELISA）检查了九十五只狗尿样品中是否存在抗 Leishmania 抗体。从健康犬中收集另外二十个尿液样本作为对照。对通过蛋白A ELISA检测为阳性的26个尿液样品进行IgG2 ELISA。通过尿液抗体系数，对二十三份发现的抗利什曼原虫抗体呈阳性的尿液样本进行了尿道中抗 Leishmania 抗体的局部检测。通过蛋白质印迹（WB）分析比较了十个尿液样本（和相应的血清样本）。在95个尿液样本中，有35个呈阳性，57个呈阴性，3个对通过蛋白A ELISA检测抗体没有结论。在尿液阳性样本中发现蛋白A和IgG2水平之间存在高度相关性。抗利什曼原虫抗体存在于患有利什曼病，尿蛋白/肌酐（U P / C）比大于1且尿沉渣正常的狗的尿液中。在尿液阳性样本中，U P / C比与抗利什曼原虫抗体水平之间存在统计学意义的相关性。通常，WB分析和尿液抗体系数表明，尿液中存在抗 Leishmania 抗体是肾小球屏障过滤功能受损的结果。然而，在某些狗中，WB分析可以解释为表明抗 Leishmania 抗体的存在在较小程度上是由尿路中的局部抗体产生引起的。尿液中抗体检测可能是一种非侵入性方法，可用于患有肾小球肾病的狗进行利什曼病诊断和预后评估。

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