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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Constitutive Expression of a Bacterial Pattern Recognition Receptor, CD14, in Human Salivary Glands and Secretion as a Soluble Form in Saliva
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Constitutive Expression of a Bacterial Pattern Recognition Receptor, CD14, in Human Salivary Glands and Secretion as a Soluble Form in Saliva

机译：细菌模式识别受体CD14在人唾液腺中的组成型表达以及唾液中可溶形式的分泌

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摘要

Saliva contains a number of proteins and glycoproteins that protect oral tissues, but little is known about the role of human saliva in innate immunity. Here we showed that human major salivary gland cells constitutively expressed a bacterial pattern recognition receptor, CD14, by immunohistochemistry. Human salivary gland cells in culture express CD14 mRNA and a 55-kDa CD14 protein in, but not on the cells, and secrete a soluble form with the same molecular mass. Human whole saliva contains a 55-kDa CD14, and the concentration of parotid saliva was 10-fold higher than whole saliva, which is comparable to that of serum CD14. Levels of CD14 in unstimulated whole and parotid saliva were unchanged before and after a meal and between unstimulated and stimulated saliva, indicating that saliva CD14 is constitutively secreted into the oral cavity. In contrast, lipopolysaccharide (LPS)-binding protein was below the detectable level. The saliva CD14 is functionally active in that it mediated the activation of CD14-lacking intestinal epithelial cells by LPS in a Toll-like receptor 4-dependent manner. These results suggested that saliva CD14 is important for the maintenance of oral health and possibly intestinal homeostasis.
机译：唾液含有许多保护口腔组织的蛋白质和糖蛋白，但人们对唾液在先天免疫中的作用知之甚少。在这里，我们显示了人类主要唾液腺细胞通过免疫组织化学组成性表达细菌模式识别受体CD14。培养的人唾液腺细胞在细胞中表达CD14 mRNA和55 kDa CD14蛋白，但在细胞中不表达，并分泌具有相同分子量的可溶形式。人的整个唾液含有55kDa的CD14，腮腺唾液的浓度比整个唾液高10倍，与血清CD14相当。饭前和饭后以及未刺激和刺激的唾液之间未刺激的全唾液和腮腺唾液中CD14的水平未改变，表明唾液CD14组成型分泌到口腔中。相反，脂多糖（LPS）结合蛋白低于可检测的水平。唾液CD14具有功能活性，因为它以Toll样受体4依赖性方式介导LPS激活了缺少CD14的肠上皮细胞。这些结果表明，唾液CD14对于维持口腔健康和可能的肠道稳态非常重要。

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