Effect of Multiple Freeze-Thaw Cycles on Detection of Measles, Mumps, and Rubella Virus Antibodies

Norman A. Pinsky; Jeanne M. Huddleston; Robert M. Jacobson; Peter C. Wollan; Gregory A. Poland

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Effect of Multiple Freeze-Thaw Cycles on Detection of Measles, Mumps, and Rubella Virus Antibodies

机译：多个冻融循环对麻疹，腮腺炎和风疹病毒抗体检测的影响

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We investigated the effect of multiple freeze-thaw cycles on mumps, measles, and rubella virus serum antibody levels with whole-virus immunoglobulin G enzyme-linked immunoassays. Fresh serum samples from nine healthy adult volunteers were divided into six sets of five aliquots each. Samples were taken through a total of 10 freeze-thaw cycles and stored at 4°C until assayed. Each assay measurement was done in replicates of five, and the mean value was reported. After completing 10 freeze-thaw cycles, we found no clinically or statistically significant effect on measured antibody levels and found no discernible detrimental effect on the ability to measure these antibodies by enzyme-linked immunoassays.

机译：我们用全病毒免疫球蛋白G酶联免疫法研究了多个冻融循环对腮腺炎，麻疹和风疹病毒血清抗体水平的影响。将来自九名健康成人志愿者的新鲜血清样本分成六组，每组五份。样品经过总共10次冻融循环后取出，并保存在4°C下直至进行分析。每次测定测量均重复五次，并记录平均值。在完成10次冻融循环后，我们发现对测量的抗体水平无临床或统计学显着影响，也未发现对通过酶联免疫法测量这些抗体的能力有明显的有害影响。

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《Clinical and diagnostic laboratory immunology》 |2003年第1期|共3页
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Norman A. Pinsky; Jeanne M. Huddleston; Robert M. Jacobson; Peter C. Wollan; Gregory A. Poland;
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1. Detection of serum antibodies against measles, mumps and rubella after primary measles, mumps and rubella (MMR) vaccination in children [J] . TabatabaeiS.R., EsteghamatiA.-R., ShivaF., Archives of Iranian medicine . 2013,第1期

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2. Detection of IgG-class antibodies to measles, mumps, rubella, and varicella-zoster virus using a multiplex bead immunoassay. [J] . Dhiman N, Jespersen DJ, Rollins LO, Diagnostic microbiology and infectious disease . 2010,第4期

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3. Dot immunobinding assay for simultaneous detection of specific immunoglobulin G antibodies to measles virus, mumps virus, and rubella virus. [J] . F Condorelli, T Ziegler Journal of Clinical Microbiology . 1993,第3期

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4. Application of semi-logarithm regression in determining the antibody level measles virus and rubella virus by indirect elisa [C] . Tang Quan, Liu Xiulan, Zheng Chunzao 7th China-Japan International Congress of Microbiology Shanghai Symposium-2000 4-6 August 2000 Shanghai . 2000

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5. The risk of idiopathic thrombocytopenic purpura (ITP) following measles, mumps, and rubella (MMR) vaccination: Attributable risk and a simulation study to evaluate four study designs. [D] . Glanz, Jason M. 2005

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6. Effect of Multiple Freeze-Thaw Cycles on Detection of Measles Mumps and Rubella Virus Antibodies [O] . Norman A. Pinsky, Jeanne M. Huddleston, Robert M. Jacobson, 2003

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7. Effect of Multiple Freeze-Thaw Cycles on Detection of Measles, Mumps, and Rubella Virus Antibodies [O] . Pinsky, Norman A., Huddleston, Jeanne M., Jacobson, Robert M., 2003

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Effect of Multiple Freeze-Thaw Cycles on Detection of Measles, Mumps, and Rubella Virus Antibodies

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