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首页> 外文期刊>Diagnostic microbiology and infectious disease >Detection of IgG-class antibodies to measles, mumps, rubella, and varicella-zoster virus using a multiplex bead immunoassay.
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Detection of IgG-class antibodies to measles, mumps, rubella, and varicella-zoster virus using a multiplex bead immunoassay.

机译：使用多重磁珠免疫测定法检测针对麻疹，腮腺炎，风疹和水痘带状疱疹病毒的IgG类抗体。

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摘要

Serologic testing for measles, mumps, rubella, and varicella (MMRV) IgG is traditionally performed by immunofluorescence assay or enzyme immunoassay (EIA). Although sensitive and specific, these methods are labor intensive, time consuming, and require separate assays for each analyte. This study evaluated the performance of the MMRV IgG AtheNA Multi-Lyte assay using nonclinically characterized serum specimens submitted to our laboratory for routine MMRV IgG testing. Mumps (n = 492) or rubella (n = 500) IgG were initially tested by enzyme-linked fluorescent antibody (ELFA), whereas measles (n = 494) or varicella (n = 497) were analyzed by EIA. Each sample was also tested by the AtheNA Multi-Lyte assay. Discordant results were retested by the predicate method and the multiplex assay, with further discrepancies being arbitrated by a third test. Compared to EIA/ELFA for MMRV IgG, the AtheNA assay demonstrated an overall agreement of 97.4%, 98.2%, 97.6%, and 100%, respectively. Use of this multiplex assay allows for the simultaneous detection of MMRV IgG, potentially decreasing cost, sample volume requirements, aliquot errors, and hands-on testing time.
机译：麻疹，腮腺炎，风疹和水痘（MMRV）IgG的血清学检测传统上是通过免疫荧光测定法或酶免疫测定法（EIA）进行的。尽管这些方法灵敏且特异，但这些方法费时费力，并且需要对每种分析物进行单独的测定。这项研究使用提交给我们实验室进行常规MMRV IgG测试的非临床特征血清标本评估了MMRV IgG AtheNA Multi-Lyte分析的性能。腮腺炎（n = 492）或风疹（n = 500）IgG最初是通过酶联荧光抗体（ELFA）测试的，而麻疹（n = 494）或水痘（n = 497）则通过EIA分析。还通过AtheNA Multi-Lyte测定法测试了每个样品。不一致的结果通过谓词方法和多重分析进行了重新测试，进一步的差异则通过第三次测试进行仲裁。与针对MMRV IgG的EIA / ELFA相比，AtheNA分析显示总体一致性分别为97.4％，98.2％，97.6％和100％。使用这种多重分析可以同时检测MMRV IgG，从而可能降低成本，减少样品量要求，分装错误和动手测试时间。

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