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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Low Levels of Antigenic Variability in Fluconazole-Susceptible and -Resistant Candida albicansIsolates from Human Immunodeficiency Virus-Infected Patients with Oropharyngeal Candidiasis
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Low Levels of Antigenic Variability in Fluconazole-Susceptible and -Resistant Candida albicansIsolates from Human Immunodeficiency Virus-Infected Patients with Oropharyngeal Candidiasis

机译：人类免疫缺陷病毒感染的口咽念珠菌病患者对氟康唑敏感和耐药的白色念珠菌分离株的抗原变异水平低

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Three serial isolates of Candida albicans were obtained by direct swab or by oral saline rinses from each of five human immunodeficiency virus-infected patients with recurrent oropharyngeal candidiasis. Genotyping techniques confirmed the presence of a persistent strain in multiple episodes from the same patient, which was different from the strains isolated from other patients. Fluconazole susceptibility was determined by both an agar dilution method and the National Committee for Clinical Laboratory Standards macrobroth procedure. In four of these patients the strains developed fluconazole resistance, and in one patient the strain remained susceptible. The different isolates were propagated as yeast cells on a synthetic medium, and their cell wall proteinaceous components were extracted by treatment with β-mercaptoethanol. Protein and mannoprotein components present in the extracts were analyzed by electrophoresis, immunoblotting, and lectin-blotting techniques. The analysis showed a similar composition, with only minor qualitative and quantitative differences in the polypeptidic and antigenic patterns associated with the cell wall extracts from serial isolates from the same patient, as well as those from different strains isolated from different patients. Use of monospecific antibodies generated against two immunodominant antigens during candidiasis (enolase and the 58-kDa fibrinogen-binding mannoprotein) demonstrated their expression in all isolates tested. Overall, the antigenic makeup of C. albicans strains remained constant during the course of infection and was not affected by development of fluconazole resistance. In contrast to previous reports, the low degree of antigenic variability observed in this study may be due to the fact that the isolates were obtained from a highly homogeneous population of patients and to the uniformity in techniques used for the isolation, storage, and culture of the different strains, as well as extraction methodologies.
机译：通过直接拭子或口服盐水冲洗分别从五名人类免疫缺陷病毒感染的复发性口咽念珠菌病患者中获得了三个白色念珠菌系列分离株。基因分型技术证实了同一患者多次发作中存在持久性菌株，这与从其他患者中分离出的菌株不同。氟康唑的药敏性通过琼脂稀释法和美国国家临床实验室标准委员会的肉汤程序确定。在这些患者中有四名菌株产生氟康唑耐药性，而在一名患者中，菌株仍然易感。将不同的分离物作为酵母细胞在合成培养基上繁殖，并通过用β-巯基乙醇处理来提取其细胞壁蛋白质成分。通过电泳，免疫印迹和凝集素印迹技术分析了提取物中存在的蛋白质和甘露糖蛋白成分。分析显示相似的组成，与来自同一患者的系列分离株的细胞壁提取物以及来自不同患者的不同菌株的细胞壁提取物相关的多肽和抗原模式在质量和数量上仅存在微小差异。在念珠菌病期间使用针对两种免疫显性抗原（烯醇酶和58 kDa纤维蛋白原结合甘露糖蛋白）的单特异性抗体的使用证明了它们在所有测试分离物中的表达。总体而言， C的抗原组成。白色念珠菌菌株在感染过程中保持恒定，不受氟康唑耐药性发展的影响。与以前的报告相比，本研究中观察到的抗原变异性程度低可能是由于分离株是从高度均一的患者群体中获得的，并且是由于分离，储存和培养的技术的一致性。不同的菌株，以及提取方法。

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