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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Seroepidemiology of Human Group C Rotavirus in Japan Based on a Blocking Enzyme-Linked Immunosorbent Assay
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Seroepidemiology of Human Group C Rotavirus in Japan Based on a Blocking Enzyme-Linked Immunosorbent Assay

机译：基于阻断酶联免疫吸附测定的日本人类C型轮状病毒血清流行病学

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A novel blocking enzyme-linked immunosorbent assay (BL-ELISA) was developed for detection of antibodies to human group C rotavirus (CHRV). The specificity of the BL-ELISA was confirmed by using animal sera hyperimmunized to group A and group C rotaviruses and paired sera from five patients with acute CHRV gastroenteritis. Furthermore, there was concordance between the BL-ELISA and a neutralization assay for CHRV in 226 (95%) of 238 samples. By using the BL-ELISA, we determined the seroprevalence of CHRV in 704 serum samples obtained from nine different age groups of inhabitants of Okayama Prefecture, Japan, in 1992, 1994, and 1996. As a result, 211 sera (30%) were found to be positive for CHRV antibodies. The seroprevalence gradually increased with age and reached 52.7% in the oldest individuals. A further analysis of the youngest age group suggested that CHRVs predominantly prevail in persons older than 3 years of age in Japan. When comparing the three sampling years, a larger percentage of antibody-positive sera was detected in 1994 than in either 1992 or 1996 in individuals between 6 and 15 years of age, reflecting the occurrence of a CHRV outbreak among children during the winter of 1992 to 1993 that was previously documented. These results indicate that CHRV infections may occur more frequently in spite of the relatively low detection rate of the virus.
机译：开发了一种新型的封闭酶联免疫吸附测定（BL-ELISA），用于检测人类C型轮状病毒（CHRV）的抗体。 BL-ELISA的特异性通过使用对A组和C组轮状病毒超免疫的动物血清以及来自5例急性CHRV肠胃炎患者的配对血清来证实。此外，在238个样品中的226个（95％）中，BL-ELISA和CHRV中和试验之间存在一致性。通过BL-ELISA，我们确定了1992年，1994年和1996年从日本冈山县9个不同年龄组的居民中获得的704个血清样本中CHRV的血清阳性率。结果，有211例血清（占30％）被发现对CHRV抗体呈阳性。血清阳性率随着年龄的增长而逐渐增加，在年龄最大的人群中达到52.7％。对最年轻年龄组的进一步分析表明，在日本3岁以上的人群中CHRV占主导地位。比较这三个采样年时，1994年检测到的抗体阳性血清百分比高于1992年或1996年的6至15岁个体，反映了1992年冬季至2003年冬季儿童CHRV暴发的发生。以前有记载的1993年。这些结果表明，尽管病毒的检出率相对较低，但CHRV感染仍可能更频繁地发生。

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