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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Lipopolysaccharide from Nonvirulent Ara+ Burkholderia pseudomallei Isolates Is Immunologically Indistinguishable from Lipopolysaccharide from Virulent Ara? Clinical Isolates
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Lipopolysaccharide from Nonvirulent Ara+ Burkholderia pseudomallei Isolates Is Immunologically Indistinguishable from Lipopolysaccharide from Virulent Ara? Clinical Isolates

机译：来自非毒性Ara +假伯克霍尔德菌分离物的脂多糖与来自毒性Ara的脂多糖在免疫学上没有区别吗？临床隔离

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摘要

Different lines of evidence suggest that a discrepancy between the distribution of Burkholderia (Pseudomonas) pseudomallei in the environment and the distribution of the disease melioidosis is attributable, at least in part, to phenotypic differences between clinical and some environmental isolates. Two antigenically and biochemically distinct biotypes have been described, only one of which is virulent. In this study, lipopolysaccharides (LPSs) were extracted by the proteinase K digestion method from a total of 214 B. pseudomallei isolates, and their immunoreactivities with sera from patients with different clinical spectra and with other infections were evaluated. With the exception of 4 isolates from a total of 214 tested, the sodium dodecyl sulfate-polyacrylamide gel electrophoresis silver-staining profiles of the LPSs from the two biotypes showed identical ladder patterns that were typical for smooth LPSs from other gram-negative bacteria. The 210 isolates with typical LPS patterns (119 Ara? clinical, 13 Ara? soil, 70 Ara+ soil, and 8 reference National Type Culture Collection strains) also exhibited similar immunoblot profiles against pooled sera from patients with melioidosis and hyperimmune mouse sera. Concordant findings were noted in the indirect enzyme-linked immunosorbent assay with Ara? and Ara+ LPSs to coat the microtiter plates. The LPSs of the different B. pseudomallei biotypes appear antigenically indistinguishable. It is, therefore, unlikely that this component is related to the virulence and pathogenicity ofB. pseudomallei.
机译：不同的证据表明，环境中伯克霍尔德氏菌（假单胞菌）的分布与假肢病的分布之间的差异至少部分归因于临床和某些环境之间的表型差异。隔离株。已经描述了两种在抗原和生物化学上不同的生物型，其中只有一种是强毒的。在这项研究中，通过蛋白酶K消化法从总共214 B中提取脂多糖（LPS）。评估了假苹果芽孢杆菌的分离物，以及它们与不同临床谱图和其他感染患者血清的免疫反应性。除了从总共测试的214种细菌中分离出4种菌株外，两种生物型LPS的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳银染图谱均显示出相同的梯形图谱，这对于来自其他革兰氏阴性细菌的平滑LPS而言是典型的。具有典型LPS模式的210个分离株（临床上为119 Ara ？土壤，13 Ara ？土壤，70 Ara + 土壤和8个参考国家典型培养物收集菌株也对来自类li虫病和超免疫小鼠血清的合并血清表现出相似的免疫印迹特性。在间接酶联免疫吸附试验中，使用Ara ？和Ara + LPSs包被微量滴定板，发现了一致的发现。不同的 B的LPS。假性马来生物型在抗原学上没有区别。因此，该成分不太可能与B的毒力和致病性有关。假苹果。

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