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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Adherence of Giardia lambliaTrophozoites to Int-407 Human Intestinal Cells
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Adherence of Giardia lambliaTrophozoites to Int-407 Human Intestinal Cells

机译：贾第鞭毛虫滋养体对Int-407人肠道细胞的粘附

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Attachment of Giardia lamblia trophozoites to enterocytes is essential for colonization of the small intestine and is considered a prerequisite for parasite-induced enterocyte dysfunction and clinical disease. In this work, coincubation of Giardiawith Int-407 cells, was used as an in vitro model to study the role of cytoskeleton and surface lectins involved in the attachment of the parasite. This interaction was also studied by scanning and transmission electron microscopy. Adherence was dependent on temperature and was maximal at 37°C. It was reduced by 2.5 mM colchicine (57%), mebendazole (10 μg/ml) (59%), 100 mM glucose (26%), 100 mM mannose (22%), 40 mM mannose-6-phosphate (18%), and concanavalin A (100 μg/ml) (21%). No significant modification was observed when Giardia was pretreated with cytochalasins B and D and with EDTA. Giardia attachment was also diminished by preincubating Int-407 cells with cytochalasin B and D (5 μg/ml) (16%) and by glutaraldehyde fixation of intestinal cells and ofG. lamblia trophozoites (72 and 100%, respectively). Ultrastructural studies showed that Giardia attaches to the Int-407 monolayer predominantly by its ventral surface. Int-407 cells contact trophozoites with elongated microvilli, and both trophozoite imprints and interactions of Giardia flagella with intestinal cells were also observed. Transmission electron microscopy showed that Giardia lateral crest and ventrolateral flange were important structures in the adherence process. Our results suggest a combination of mechanical and hydrodynamic forces in trophozoite attachment; surface lectins also seem to mediate binding and may be involved in specific recognition of host cells.
机译：小肠贾第鞭毛虫滋养体附着在肠细胞上对于小肠定植是必不可少的，并且被认为是寄生虫诱发的肠细胞功能障碍和临床疾病的先决条件。在这项工作中，将贾第鞭毛虫与Int-407细胞共孵育用作体外模型，以研究细胞骨架和表面凝集素在寄生虫附着中的作用。还通过扫描和透射电子显微镜研究了这种相互作用。附着力取决于温度，在37°C时最大。降低了2.5 mM秋水仙碱（57％），甲苯咪唑（10μg/ ml）（59％），100 mM葡萄糖（26％），100 mM甘露糖（22％），40 mM 6-磷酸甘露糖（18％） ）和伴刀豆球蛋白A（100μg/ ml）（21％）。用细胞松弛素B和D以及EDTA预处理 Giardia 时，未观察到明显的改变。通过用细胞松弛素B和D（5μg/ ml）（16％）预孵育Int-407细胞，以及肠细胞和 G的戊二醛固定作用，也可以减少 Giardia 的附着。兰布里亚滋养体（分别为72％和100％）。超微结构研究表明，主要通过腹面附着在Int-407单层上。 Int-407细胞与滋养体接触带有细长的微绒毛，还观察到滋养体印记和鞭毛鞭毛虫与肠道细胞的相互作用。透射电子显微镜显示，贾第鞭毛虫的侧lateral和腹侧突缘是粘附过程中的重要结构。我们的结果表明，在滋养体附着过程中，机械力和流体动力相结合。表面凝集素似乎也介导结合，并且可能参与宿主细胞的特异性识别。

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