...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Clinical and diagnostic laboratory immunology >Detection of West Nile Virus (WNV)-Specific Immunoglobulin M in a Reference Laboratory Setting during the 2002 WNV Season in the United States
【24h】

Detection of West Nile Virus (WNV)-Specific Immunoglobulin M in a Reference Laboratory Setting during the 2002 WNV Season in the United States

机译：在美国2002 WNV季节的参考实验室环境中检测特定于西尼罗河病毒（WNV）的免疫球蛋白M

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Between 1 June and 31 December 2002, 30,677 serum samples and 4,554 cerebrospinal fluid (CSF) samples were tested for West Nile virus (WNV)-specific immunoglobulin M (IgM) by an in-house enzyme-linked immunosorbent assay (ELISA); 1,481 serum samples (4.8%) and 345 CSF samples (7.6%) were positive for WNV IgM. Positive samples were forwarded to public health service laboratories (PHSLs) for further testing. PHSLs supplied results from their WNV IgM ELISAs for 654 samples; 633 (97%) were positive. PHSLs supplied WNV plaque reduction neutralization test results for 128 samples; 123 (96%) were positive. WNV IgM seroconversion and seroreversion trends were evaluated for 749 patients who each provided two serum samples that were tested during the study period. Of 574 patients whose first serum sample was IgM negative, 41 (7%) seroconverted (the second serum sample was IgM positive); of 175 patients whose first serum sample was IgM positive, 22 (13%) seroreverted (the second serum sample was IgM negative). The seroreversion rate was directly proportional to the time between serum sample collection; whereas only 1% of patients whose sera were collected <20 days apart showed seroreversion, 54% of patients whose sera were collected >60 days apart showed seroreversion. Conversion and reversion trends for CSF were evaluated for 68 patients. Of 54 patients whose first CSF specimen was IgM negative, 9 (17%) converted; none of 14 patients whose first CSF specimen was IgM positive reverted. Concomitant detection of WNV IgM in serum and CSF was assessed for 1,188 patients for whom paired serum and CSF specimens were available; for all 130 patients for whom IgM was detectable in CSF, IgM was also detectable in serum. These findings show that an in-house WNV IgM ELISA accurately identifies patients with WNV infection, document WNV IgM conversion and reversion trends, and demonstrate that WNV IgM detection in CSF is accompanied by WNV IgM detection in serum.
机译：在2002年6月1日至12月31日期间，通过内部酶联免疫吸附试验（ELISA）对30,677份血清样本和4,554份脑脊液（CSF）样本进行了西尼罗河病毒（WNV）特异性免疫球蛋白M（IgM）的检测; 1,481份血清样本（4.8％）和345份CSF样本（7.6％）的WNV IgM呈阳性。阳性样本被转发到公共卫生服务实验室（PHSL）进行进一步测试。 PHSL提供了654个样品的WNV IgM ELISA结果; 633（97％）为阳性。 PHSL提供了128个样品的WNV斑块减少中和测试结果; 123（96％）为阳性。对749例患者的WNV IgM血清转化和血清逆转趋势进行了评估，每个患者均提供了两个在研究期间进行测试的血清样本。在574例患者的第一份血清样本IgM阴性的患者中，有41例（7％）发生血清转化（第二份血清样本IgM阳性）。在175名患者的第一份血清样本中IgM阳性的患者中，有22名（13％）被血清去除（第二份血清样本中IgM阴性）。血清逆转率与血清样品收集之间的时间成正比;而仅在间隔时间少于20天的患者中有1％出现血清逆转，而间隔时间超过60天的患者中有54％出现血清逆转。评价了68例患者的CSF转换和逆转趋势。在54例首例脑脊液IgM阴性的患者中，有9例（17％）转换; 14例首例CSF标本为IgM阳性的患者均未恢复。评估了血清和脑脊液中WNV IgM的同时检测，共有1188例患者可获得血清和脑脊液标本配对。对于在脑脊液中可检测到IgM的所有130位患者，在血清中也可检测到IgM。这些发现表明，内部WNV IgM ELISA可以准确识别WNV感染患者，记录WNV IgM转化和逆转趋势，并证明CSF中WNV IgM检测与血清中WNV IgM检测伴随。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号