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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Murine splenic hematopoietic subpopulations: the enlarged undifferentiated subset in New Zealand black mice is multipotent stem cells.
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Murine splenic hematopoietic subpopulations: the enlarged undifferentiated subset in New Zealand black mice is multipotent stem cells.

机译：小鼠脾脏造血亚群：新西兰黑小鼠中扩大的未分化亚群是多能干细胞。

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We recently reported that a significant population of the murine splenic non-T, non-B "null" cell compartment consists of non-lineage-specific, undifferentiated cells which are in the G0 and G1 phases of the cell cycle and that their numbers are particularly high in the spleens of New Zealand Black mice. A highly enriched population of these non-lineage-specific cells obtained by successive elimination of differentiated cells was further purified to homogeneity by fluorescence-activated cell sorting. The morphologic, phenotypic, and histochemical characteristics of this purified population suggest that these cells may be primitive hematopoietic stem cells. The germ line configuration of the genomic DNA establishes that these are uncommitted stem cells. In vivo, these cells form day 12 colonies in the spleen and liver of lethally irradiated recipients and confer radioprotection. These cells also differentiate into T- and B-cell lineages and reconstitute the immunodeficiency in mice with severe combined immunodeficiency. In response to a combination of a very few early-acting lymphokines and/or stromal cell-conditioned medium in vitro, these cells differentiate into both myeloid and lymphoid cell types. More of these cells are obtained from the enlarged spleens of New Zealand Black mice than from those of BALB/c mice. The presence of a comparatively higher number of stem cells in the spleen than in the marrow or fetal liver provides an alternative, and possibly superior, source of uncommitted stem cells for a variety of experimental investigations or therapeutic manipulations.
机译：我们最近报道，鼠脾脏的非T，非B“空”细胞区隔中有大量细胞是由非谱系特异性，未分化的细胞组成，它们处于细胞周期的G0和G1期，其数量为在新西兰黑老鼠的脾脏中特别高。通过连续消除分化细胞而获得的这些非谱系特异性细胞的高度富集的群体通过荧光激活的细胞分选进一步纯化至同质。此纯化种群的形态，表型和组织化学特征表明，这些细胞可能是原始的造血干细胞。基因组DNA的种系构型确定这些是未定型的干细胞。在体内，这些细胞在接受致死性照射的受者的脾脏和肝脏中形成第12天的菌落，并具有放射防护作用。这些细胞还可分化为T细胞和B细胞谱系，并在具有严重综合免疫缺陷的小鼠中重建免疫缺陷。在体外响应极少数早期作用的淋巴因子和/或基质细胞条件培养基的组合时，这些细胞分化为髓样和淋巴样细胞类型。从BALB / c小鼠的脾脏中，更多的是从新西兰黑小鼠的脾脏中获得的。脾脏中的干细胞数量比骨髓或胎儿肝脏中的干细胞数量相对多，这为各种实验研究或治疗操作提供了另一种可能，而且可能是更好的未定型干细胞来源。

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