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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Human antibody reactivity against the lower matrix protein (pp65) produced by cytomegalovirus.
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Human antibody reactivity against the lower matrix protein (pp65) produced by cytomegalovirus.

机译：人类抗体对巨细胞病毒产生的下部基质蛋白（pp65）具有反应性。

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The lower matrix protein (pp65) is a major product of many laboratory strains of cytomegalovirus (CMV). It is thus an integral part of many CMV serological assays based on native antigen. Recombinant fragments of pp65 have previously been investigated for their usefulness in more-defined assays. The latter antigens have, however, failed to develop a positive response with serum samples derived from a substantial number of infected individuals. Here we show that the human humoral immune response to CMV pp65 is highly diverse and recognizes at least seven distinct but in some cases partly overlapping epitopes. Most of these epitopes could not be mimicked by any of the investigated recombinant or synthetic antigens. Furthermore, when we investigated the ability of human CMV-seropositive serum samples to block the reactivity of pp65-specific antibodies recognizing five different epitopes within pp65, it was evident that several sera did not contain significant levels of antibodies against any of these or overlapping structures. It was thus concluded that the antibody response against CMV pp65 is weak in some CMV-infected individuals, making this antigen unsuitable for use alone in serological screening systems for CMV infection.
机译：下基质蛋白（pp65）是许多巨细胞病毒（CMV）实验室菌株的主要产物。因此，它是许多基于天然抗原的CMV血清学检测不可或缺的部分。以前已经研究了pp65重组片段在更明确的测定中的有用性。然而，后一种抗原未能对源自大量感染个体的血清样品产生阳性反应。在这里，我们显示人类对CMV pp65的体液免疫反应高度多样，并识别至少七个不同但在某些情况下部分重叠的表位。这些表位中的大多数不能被任何研究的重组或合成抗原所模仿。此外，当我们研究人CMV血清阳性血清样本阻断识别pp65内五种不同表位的pp65特异性抗体的反应能力时，很明显，几种血清不含有针对这些或重叠结构中任何一种的显着水平的抗体。因此得出结论，在一些CMV感染的个体中，针对CMV pp65的抗体应答较弱，使得该抗原不适合单独用于CMV感染的血清学筛选系统。

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