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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Validation of a Gastrointestinal Explant System for Measurement of Mucosal Antibody Production
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Validation of a Gastrointestinal Explant System for Measurement of Mucosal Antibody Production

机译：用于测量粘膜抗体生产的胃肠外植体系统的验证

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A gastrointestinal explant culture system was developed and compared to the mononuclear cell extraction and enzyme-linked immunospot assay method for measurement of immunoglobulin A (IgA) and IgG antibody-secreting cells (ASCs) in gastric antral and duodenal biopsies of non-Helicobacter pylori-infected volunteers. IgA and IgG were detected in explant supernatants during 6 to 7 days of culture in all subjects. IgA containing secretory component was also detected throughout the culture period, although peak production occurred only in the first 3 days. During 7 days of culture, the cumulative geometric mean IgA levels produced were 2.2 and 8.02 μg/ml/10 mg of antral and duodenal biopsy tissues, respectively, while the cumulative geometric mean IgG levels were 1.54 and 2.92 μg/ml/10 mg of antral and duodenal biopsy tissues, respectively. Cycloheximide treatment resulted in a >90% reduction in both immunoglobulin classes after 6 days of treatment compared to levels in untreated controls. The detection of IgA and IgG ASCs extracted from biopsies on days 1 and 6 of culture confirmed that the antibody detected was derived from mucosal lamina propria. The IgA and IgG ASC responses were positively correlated with antibody concentrations detected in culture supernatants (r = 0.87 and 0.85, respectively). These results validate the potential usefulness of our gastrointestinal explant system for the evaluation of mucosal effector B-cell function.
机译：开发了胃肠外植体培养系统，并将其与单核细胞提取和酶联免疫斑点测定法进行比较，以测量非的胃窦和十二指肠活检中的免疫球蛋白A（IgA）和IgG抗体分泌细胞（ASC）幽门螺杆菌感染的志愿者。在所有受试者的培养6至7天期间，在外植体上清液中检测到IgA和IgG。在整个培养期间也检测到含有分泌成分的IgA，尽管仅在头3天就产生了峰值。在培养的7天中，所产生的累积几何平均IgA水平分别为十二指肠和十二指肠活检组织的2.2和8.02μg/ ml / 10 mg，而累积的IgG平均几何平均水平分别为1.54和2.92μg/ ml / 10 mg分别为肛门和十二指肠活检组织。与未处理的对照组相比，用环己酰亚胺治疗6天后，两种免疫球蛋白类别的减少幅度均> 90％。在培养的第1天和第6天检测到从活检组织中提取的IgA和IgG ASC，证实检测到的抗体来源于固有层黏膜。 IgA和IgG ASC反应与培养上清液中检测到的抗体浓度呈正相关（分别为 r = 0.87和0.85）。这些结果验证了我们的胃肠外植体系统对粘膜效应B细胞功能的评估的潜在有用性。

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