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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Expression and Self-Assembly of Grimsby Virus: Antigenic Distinction from Norwalk and Mexico Viruses
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Expression and Self-Assembly of Grimsby Virus: Antigenic Distinction from Norwalk and Mexico Viruses

机译：格里姆斯比病毒的表达和自组装：诺沃克和墨西哥病毒的抗原区别。

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A cDNA obtained from Grimsby virus (GRV), a Norwalk-like virus, purified from a stool sample of a symptomatic adult associated with a gastroenteritis outbreak in the United Kingdom, was used to obtain the complete nucleotide sequence of the second open reading frame (ORF2). The ORF2 sequence of GRV predicts a capsid of 539 amino acids (aa) which exhibits aa identities of 96% to Lordsdale virus, 67% to Mexico virus (MXV), and 43% to Norwalk virus (NV). The GRV capsid protein was expressed in insects cells by using a recombinant baculovirus, and the resulting virus-like particles (VLPs) possessed a protein with an apparent molecular weight of 58,000. Hyperimmune antisera raised against purified GRV, MXV, and NV VLPs were tested in an indirect enzyme-linked immunosorbent assay (ELISA) against GRV, NV, and MXV VLPs, revealing that GRV is antigenically distinct from both NV and MXV. The antigenic specificity of the GRV-hyperimmune antiserum was confirmed in an antigen capture ELISA using GRV-, NV-, or MXV-containing fecal specimens. The expression of the GRV capsid protein has, for the first time, allowed the antigenic comparison of three distinct recombinant Norwalk-like viruses.
机译：从英国与胃肠炎暴发有关的有症状成人粪便样本中纯化的，从格里姆斯比病毒（GRV）（一种诺沃克样病毒）中获得的cDNA用于获得第二个开放阅读框的完整核苷酸序列（ ORF2）。 GRV的ORF2序列可预测衣壳为539个氨基酸（aa），与洛兹代尔病毒，氨基酸对墨西哥病毒（MXV）的氨基酸同一性分别为96％，67％和43％。通过使用重组杆状病毒在昆虫细胞中表达GRV衣壳蛋白，并且所得的病毒样颗粒（VLP）具有表观分子量为58,000的蛋白。在针对GRV，NV和MXV VLP的间接酶联免疫吸附测定（ELISA）中测试了针对纯化的GRV，MXV和NV VLP产生的超免疫抗血清，显示GRV在抗原上不同于NV和MXV。使用含有GRV，NV或MXV的粪便标本在抗原捕获ELISA中证实了GRV超免疫抗血清的抗原特异性。 GRV衣壳蛋白的表达首次允许对三种不同的重组诺沃克样病毒进行抗原比较。

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