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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Epitope-Specific Antibody Levels Demonstrate Recognition of New Epitopes and Changes in Titer but Not Affinity during Treatment of Tuberculosis
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Epitope-Specific Antibody Levels Demonstrate Recognition of New Epitopes and Changes in Titer but Not Affinity during Treatment of Tuberculosis

机译：表位特定的抗体水平证明了结核病治疗过程中新表位的识别和滴度变化但非亲和力变化

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Antibody levels rise during treatment of tuberculosis. This study examined when this rise occurred, whether there was recognition of new antigen binding sites (epitopes) on the same or different antigens, and how long specific antibody persisted. Forty patients with smear-positive pulmonary tuberculosis provided serum before and during treatment. Antibody levels were measured using a monoclonal antibody competition assay to epitopes restricted to the Mycobacterium tuberculosis complex and an enzyme-linked immunosorbent assay for lipoarabinomannan. Significant increases in antibody levels were apparent after 7 days of treatment. Five samples (12.5%) had positive titers to all epitopes at the start of treatment, and this increased to 23 (58%) during treatment. Antibody to epitopes with the poorest sensitivity (the TB23 epitope of the 19-kDa antigen and the TB78 epitope of hsp65) showed the greatest increases after treatment. Antibody to these two epitopes was also absent in some patients with relapsed tuberculosis until after treatment. Antibody titers showed a biphasic response, with a fall at 2 to 3 months of treatment. Sera from two patients showed changes in the affinity of epitope-specific antibody during treatment, whereas the majority did not. Those infected with isoniazid-resistant strains of M. tuberculosis showed a late rise in antibody. Antibody to the TB68 epitope of the 16-kDa α-crystallin homolog was short-lived, but it recurred with bacteriological relapse during treatment. Positive antibody titers persisted for at least 3 to 18 months after treatment. Diagnostic tests for tuberculosis should be evaluated using only pretreatment sera. Delayed antigenic recognition could be due to active suppression and/or failure to engage internal antigens of M. tuberculosis.
机译：结核病治疗期间抗体水平升高。这项研究检查了何时出现这种上升，是否在相同或不同抗原上识别出新的抗原结合位点（表位），以及特异性抗体持续了多长时间。四十名涂阳肺结核患者在治疗前和治疗期间提供血清。使用针对结核分枝杆菌复合物的抗原决定簇的单克隆抗体竞争测定法和脂阿拉伯甘露聚糖酶联免疫吸附法测定抗体水平。治疗7天后，抗体水平明显增加。在治疗开始时，有五个样品（12.5％）对所有表位的滴度均为阳性，在治疗过程中增加至23个（58％）。具有最差敏感性的抗原决定簇的抗体（19 kDa抗原的TB23抗原决定簇和hsp65的TB78抗原决定簇）在治疗后表现出最大的增加。在一些结核复发患者中，直到治疗后，这两个表位的抗体也缺失。抗体滴度显示出双相反应，在治疗2至3个月时下降。两名患者的血清在治疗过程中表位特异性抗体的亲和力发生了变化，而大多数患者则没有。那些感染了耐异烟肼的 M菌株的人。结核病显示抗体晚期升高。 16-kDaα-晶状蛋白同系物的TB68表位的抗体是短暂的，但在治疗过程中会随着细菌的复发而复发。治疗后抗体阳性滴度持续至少3至18个月。结核病的诊断测试应仅使用预处理血清进行评估。延迟的抗原识别可能是由于主动抑制和/或未能参与 M的内部抗原。结核病。

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