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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Antibody response to Prevotella spp. in patients with ventilator-associated pneumonia.
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Antibody response to Prevotella spp. in patients with ventilator-associated pneumonia.

机译：对普氏杆菌的抗体反应。呼吸机相关性肺炎的患者。

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Although anaerobic bacteria are frequently isolated from the oropharyngeal flora, their potential pathogenic role in ventilator-associated pneumonia (VAP) has been poorly investigated. In order to evaluate the pathogenic role of Prevotella spp. isolated from protected specimen brushes, we investigated the systemic humoral response with the enzyme-linked immunosorbent assay (ELISA) and Western blot (immunoblot) in 13 patients who developed a VAP associated with Prevotella species (group I). The antigen used was a mixture of whole-cell proteins taken from four reference Prevotella strains. We compared the antibody levels observed in these patients with those measured in 30 patients who developed a VAP unrelated to anaerobic bacteria (group II), in 27 patients with dental stumps (group III), and in 30 healthy patients (group IV) who had Prevotella species on dental plaque. The ELISA levels obtained in the four groups showed significant differences between group I and each of the three control groups (P < 0.05). The antibody profiles obtained by Western blot showed an intensity of response roughly superposable over levels obtained by ELISA and a species specificity. These findings suggested that colonization of these patients with Prevotella species may have been associated with an infectious process leading to a systemic humoral response and that these bacteria could play a role in VAP.
机译：尽管厌氧细菌经常从口咽菌群中分离出来，但它们在呼吸机相关性肺炎（VAP）中的潜在致病作用尚未得到充分研究。为了评估普氏杆菌的致病作用。从受保护的标本刷中分离，我们用酶联免疫吸附测定（ELISA）和Western印迹（immunoblot）研究了13例与普氏杆菌属相关的VAP的患者的全身性体液反应（I组）。所使用的抗原是取自四种参考普氏菌菌株的全细胞蛋白的混合物。我们将这些患者中观察到的抗体水平与30例发生与厌氧菌无关的VAP的患者（II组），27例有牙残端的患者（III组）和30例健康患者（IV组）进行了比较牙菌斑上的普雷沃氏菌物种。在四组中获得的ELISA水平在I组与三个对照组中的每一个之间显示出显着差异（P <0.05）。通过蛋白质印迹获得的抗体图谱显示，其反应强度与通过ELISA获得的水平大致可叠加，并且具有物种特异性。这些发现表明，这些普雷沃氏菌属患者的定植可能与导致全身性体液反应的感染过程有关，并且这些细菌可能在VAP中起作用。

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