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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Calcium Phosphate Nanoparticles Induce Mucosal Immunity and Protection against Herpes Simplex Virus Type 2
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Calcium Phosphate Nanoparticles Induce Mucosal Immunity and Protection against Herpes Simplex Virus Type 2

机译：磷酸钙纳米颗粒可诱导粘膜免疫并抵抗2型单纯疱疹病毒

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Previously we reported that calcium phosphate nanoparticles (CAP) represented a superior alternative to alum adjuvants in mice immunized with viral protein. Additionally, we showed that CAP was safe and elicited no detectable immunoglobulin E (IgE) response. In this study, we demonstrated that following mucosal delivery of herpes simplex virus type 2 (HSV-2) antigen with CAP, CAP adjuvant enhanced protective systemic and mucosal immunity versus live virus. Mice were immunized intravaginally and intranasally with HSV-2 protein plus CAP adjuvant (HSV-2+CAP), CAP alone, phosphate-buffered saline, or HSV-2 alone. HSV-2+CAP induced HSV-specific mucosal IgA and IgG and concurrently enhanced systemic IgG responses. Our results demonstrate the potency of CAP as a mucosal adjuvant. Furthermore, we show that systemic immunity could be induced via the mucosal route following inoculation with CAP-based vaccine. Moreover, neutralizing antibodies were found in the sera of mice immunized intranasally or intravaginally with HSV-2+CAP. Also, the results of our in vivo experiments indicated that mice vaccinated with HSV-2+CAP were protected against live HSV-2 infection. In conclusion, these preclinical data support the hypothesis that CAP may be an effective mucosal adjuvant that protects against viral infection.
机译：以前我们报道过磷酸钙纳米颗粒（CAP）在用病毒蛋白免疫的小鼠中代表了明矾佐剂的优良替代品。此外，我们表明CAP是安全的，不会引起可检测的免疫球蛋白E（IgE）反应。在这项研究中，我们证明了在使用CAP黏膜递送2型单纯疱疹病毒（HSV-2）抗原后，CAP佐剂增强了对活病毒的全身和黏膜免疫保护。用HSV-2蛋白加CAP佐剂（HSV-2 + CAP），单独的CAP，单独的磷酸盐缓冲液或单独的HSV-2对小鼠进行阴道内和鼻内免疫。 HSV-2 + CAP诱导HSV特异性粘膜IgA和IgG，并同时增强全身性IgG反应。我们的结果证明了CAP作为粘膜佐剂的效力。此外，我们表明接种基于CAP的疫苗后可通过粘膜途径诱导全身免疫。而且，在用HSV-2 + CAP经鼻内或阴道内免疫的小鼠的血清中发现了中和抗体。同样，我们体内实验的结果表明，接种了HSV-2 + CAP的小鼠受到了活HSV-2感染的保护。总之，这些临床前数据支持以下假设：CAP可能是一种有效的粘膜佐剂，可预防病毒感染。

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