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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Antigenicity of cell wall mannans of Candida albicans NIH B-792 (serotype B) strain cells cultured at high temperature in yeast extract-containing sabouraud liquid medium.
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Antigenicity of cell wall mannans of Candida albicans NIH B-792 (serotype B) strain cells cultured at high temperature in yeast extract-containing sabouraud liquid medium.

机译：在含酵母提取物的sabouraud液体培养基中高温培养的白色念珠菌NIH B-792（血清型B）菌株细胞的细胞壁甘露聚糖的抗原性。

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Cultivation of Candida albicans NIH B-792 (serotype B) at high temperature (37 degrees C) for 48 h in yeast extract-containing Sabouraud liquid medium (YSLM) provided the following findings in comparison with the findings obtained after incubation at 27 degrees C. Growth of the blastoconidia of this strain was decreased, with a dry weight of 9%, and the cells were deficient in cytokinesis. The cells did not undergo agglutination with serum factor 5 from a commercially available serum factor kit (Candida Check). Mannan (B-37-M) obtained from the cells cultured at 37 degrees C had partially lost its reactivity against serum factor 4 and lost most of its reactivity against serum factor 5 in an enzyme-linked immunosorbent assay (ELISA) in contrast to that (B-27-M) at 27 degrees C. Both cells and mannan prepared by cultivation first at 37 degrees C and then at 27 degrees C entirely recovered their reactivities with serum factors 4 and 5. 1H-nuclear magnetic resonance analysis also revealed that B-37-M had lost a beta-1,2-linked mannopyranose unit and retained a phosphate group. Similar changes were observed in the three other serotype B strains used in the study. The beta-1,2-linked mannooligosaccharides longer than mannotetraose were not included among the products released from B-37-M by mild acid treatment. The results of the inhibition ELISA with a series of beta-1,2-linked mannooligosaccharides from biose to octaose (M2 to M8, respectively) showed that the reactivity against serum factor 4 was inhibited most strongly by the oligosaccharides M4 to M8 and that the reactivity against serum factor 5 was inhibited completely by relatively longer oligosaccharides, M5 to M8, indicating their participation as the antigenic factor 5 epitopes.
机译：与含酵母提取物的Sabouraud液体培养基（YSLM）在高温（37摄氏度）下培养白色念珠菌NIH B-792（血清型B）48小时相比，在27摄氏度温育后获得的发现有以下发现该菌株的胚芽孢杆菌的生长减少，干重为9％，并且细胞缺乏胞质分裂。细胞未用市售血清因子试剂盒（Candida Check）中的血清因子5进行凝集。与之相比，在37℃培养的细胞中获得的甘露聚糖（B-37-M）部分失去了对血清因子4的反应性，失去了大部分对血清因子5的反应性。 （B-27-M）在27摄氏度下进行。首先在37摄氏度下，然后在27摄氏度下进行培养制备的细胞和甘露聚糖都完全恢复了它们与血清因子4和5的反应性。1H核磁共振分析还表明B-37-M失去了一个与β-1,2-连接的甘露吡喃糖单元，并保留了一个磷酸基团。在研究中使用的其他三种B型血清型菌株中观察到了类似的变化。比B-37-M通过温和酸处理释放的产品中不包括比Mannotetraose长的β-1,2-连接的甘露寡糖。一系列从生物酶到八糖（分别从M2到M8）的β-1,2-连接的甘露寡糖的抑制ELISA结果表明，寡糖M4到M8对血清因子4的反应具有最强的抑制作用。相对较长的寡糖M5至M8完全抑制了对血清因子5的反应，表明它们作为抗原性因子5表位参与。

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