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首页> 外文期刊>Comparative Hepatology >Effects of redox cycling compounds on DT diaphorase activity in the liver of rainbow trout (Oncorhynchus mykiss)
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Effects of redox cycling compounds on DT diaphorase activity in the liver of rainbow trout (Oncorhynchus mykiss)

机译：氧化还原循环化合物对虹鳟鱼肝脏中DT心肌黄递酶活性的影响

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Background DT diaphorase (DTD; NAD(P)H:quinone oxidoreductase; EC 1.6.99.2) catalyses the two electron reduction of quinones, thus preventing redox cycling and consequently quinone dependent production of reactive oxygen species. In rat and mouse, a wide range of chemicals including polyaromatic hydrocarbons, azo dyes and quinones induces DTD. Bifunctional compounds, such as β-naphthoflavone (β-NF) and benzo(a)pyrene (B(a)P), induce DTD together with CYP1A and phase II enzymes by a mechanism involving the aryl hydrocarbon receptor (AHR). Monofunctional induction of DTD is mediated through the antioxidant response element and does not lead to the induction of AHR dependent enzymes, such as CYP1A. The aim of this study was to investigate the effects of prooxidants (both bifunctional and monofunctional) on the activity of hepatic DTD in rainbow trout (Oncorhynchus mykiss) in order to evaluate DTD suitability as a biomarker. We also investigated the effect of β-NF on hepatic DTD activity in perch (Perca fluviatilis), shorthorn sculpin (Myoxocephalus scorpius), eelpout (Zoarces viviparus), brown trout (Salmo trutta) and carp (Cyprinus carpio). In addition, the effect of short term exposure to prooxidants on catalase activity was investigated. Results In rainbow trout, hepatic DTD activity is induced by the bifunctional AHR agonists β-NF and B(a)P and the monofunctional inducers naphthazarin, menadione and paraquat. Although exposure to both B(a)P and β-NF led to a strong 7-ethoxyresorufin-O-deethylase (EROD) induction, none of the monofunctional compounds affected the rainbow trout EROD activity. DTD was not induced by β-NF in any of the other fish species. Much higher DTD activities were observed in rainbow trout compared to the other fish species. Catalase activity was less responsive to short term exposure to prooxidants compared to DTD. Conclusion Since rainbow trout hepatic DTD activity is inducible by both monofunctional and bifunctional inducers, it is suggested that rainbow trout DTD may be regulated by the same mechanisms, as in mammals. The fact that DTD is inducible in rainbow trout suggests that the enzyme may be suitable as a part of a biomarker battery when rainbow trout is used in environmental studies. It appears as if DTD activity in rainbow trout is higher and inducible compared to the other fish species studied.
机译：背景DT心肌黄递酶（DTD; NAD（P）H：醌氧化还原酶; EC 1.6.99.2）催化醌的两个电子还原反应，从而防止了氧化还原循环并因此阻止了醌依赖的活性氧的产生。在大鼠和小鼠中，包括聚芳烃，偶氮染料和醌在内的多种化学物质均可诱发DTD。 β-萘黄酮（β-NF）和苯并（a）re（B（a）P）等双功能化合物通过涉及芳烃受体（AHR）的机制与CYP1A和II期酶一起诱导DTD。 DTD的单功能诱导是通过抗氧化剂反应元件介导的，不会导致AHR依赖性酶（例如CYP1A）的诱导。这项研究的目的是调查在虹鳟鱼（Oncorhynchus mykiss）中促氧化剂（双功能和单功能）对肝DTD活性的影响，以评估DTD作为生物标记物的适用性。我们还研究了β-NF对鲈鱼（Perca fluviatilis），短角刺鱼（Myoxocephalus scorpius），海豚（Zoarces viviparus），褐鳟（Salmo trutta）和鲤鱼（Cyprinus carpio）肝脏DTD活性的影响。另外，研究了短期暴露于前氧化剂对过氧化氢酶活性的影响。结果在虹鳟鱼中，双功能AHR激动剂β-NF和B（a）P以及单功能性萘，萘甲酮和百草枯可诱导肝DTD活性。尽管同时暴露于B（a）P和β-NF会导致强烈的7-乙氧基间苯二酚-O-脱乙基酶（EROD）诱导，但是没有一种单官能化合物会影响虹鳟鱼EROD活性。在其他任何鱼类中，β-NF均未引起DTD。与其他鱼类相比，虹鳟鱼的DTD活性更高。与DTD相比，过氧化氢酶活性对短期暴露于原氧化剂的反应较小。结论由于虹鳟鱼肝DTD的活性可以被单功能和双功能诱导剂诱导，因此表明虹鳟鱼DTD的调控机制可能与哺乳动物相同。 DTD可在虹鳟鱼中诱导的事实表明，当在环境研究中使用虹鳟鱼时，该酶可能适合作为生物标志物电池的一部分。与其他研究的鱼类相比，虹鳟鱼的DTD活性似乎更高且可诱导。

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