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首页> 外文期刊>Contrast media & molecular imaging >PECAMa??1a??targeted microna??sized particles of iron oxide as MRI contrast agent for detection of vascular remodeling after cerebral ischemia
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PECAMa??1a??targeted microna??sized particles of iron oxide as MRI contrast agent for detection of vascular remodeling after cerebral ischemia

机译：PECAMa？1a？靶向微粉的氧化铁颗粒作为MRI造影剂，用于检测脑缺血后的血管重塑

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An increasing amount of studies have provided evidence for vascular remodeling, for example, angiogenesis, after cerebral ischemia, which may play a significant role in posta??stroke brain plasticity and recovery. Molecular imaging can provide unique in vivo wholea??brain information on alterations in the expression of specific endothelial markers. A possible target for molecular magnetic resonance imaging (MRI) of posta??stroke (neo)vascularization is platelet endothelial cell adhesion moleculea??1 (PECAMa??1). Here we describe significantly increased PECAMa??1 mRNA levels in ipsilesional brain tissue at 6 h, 24 h and 3 days after transient middle cerebral artery occlusion in mice, and elevated PECAMa??1 staining throughout the lesion at 3, 7 and 21 days posta??stroke. The potential of microna??sized particles of iron oxide (MPIO) conjugated with PECAMa??1a??targeted antibodies, that is, ?±PECAMa??1a??MPIO, to expose strokea??induced PECAMa??1 upregulation with molecular MRI was assessed. In vitro studies demonstrated that PECAMa??1a??expressing brain endothelial cells could be effectively labeled with ?±PECAMa??1a??MPIO, giving rise to a fourfold increase in MRI relaxation rate R2. Injection of neara??infrared fluorescent dyea??labeled ?±PECAMa??1 showed target specificity and dose efficiency of the antibody for detection of brain endothelial cells at 3 days posta??stroke. However, in vivo molecular MRI at 3 and 7 days after stroke revealed no ?±PECAMa??1a??MPIOa??based contrast enhancement, which was corroborated by the absence of ?±PECAMa??1a??MPIO in post mortem brain tissue. This indicates that this molecular MRI approach, which has been proven successful for in vivo detection of other types of cell adhesion molecules, is not invariably effective for MRIa??based assessment of strokea??induced alterations in expression of cerebrovascular markers. Copyright ?? 2013 John Wiley & Sons, Ltd.
机译：越来越多的研究为脑缺血后血管重塑提供了证据，例如血管新生，这可能在卒中后脑可塑性和恢复中起重要作用。分子成像可以提供有关特定内皮标记物表达变化的独特的体内全脑信息。卒中后（新）血管形成的分子磁共振成像（MRI）的可能靶点是血小板内皮细胞粘附分子αα1（PECAMaβ1）。在这里，我们描述了小鼠短暂性中脑动脉闭塞后6 h，24 h和3天，同侧脑组织中PECAMa ?? 1 mRNA的水平显着增加，并且在整个病变3、7和21天，PECAMa ?? 1染色的升高邮政？中风。与PECAMa ?? 1a ??靶向的抗体（即？±PECAMa ?? 1a ?? MPIO）偶联的微米级氧化铁（MPIO）微粒暴露出中风诱导的PECAMa ?? 1上调的潜力。评估了分子MRI。体外研究表明，表达PECAMa 12a 1a 18的脑内皮细胞可以用α±PECAMa 12a 1a 18 MPIO有效地标记，从而使MRI弛豫率R 2增加四倍。在卒中后3天，注射标记为α±PECAMa 12的近α-红外荧光染料显示出该抗体的靶特异性和剂量效率，以检测脑内皮细胞。然而，中风后第3天和第7天的体内分子MRI显示没有基于β±PECAMaβ1aβMPIOaβ的对比增强，这被验尸后脑中没有β±PECAMaβ1aβMPIO的存在所证实。组织。这表明该分子MRI方法已被证明可成功地体内检测其他类型的细胞粘附分子，但对于基于MRIa-14的中风诱导的脑血管标志物表达改变的评估并非总是有效的。版权?? 2013 John Wiley＆Sons，Ltd.

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